Bond strength of composite resin containing biomaterial S-PRG to eroded dentin


  • Alessandra Sanchez Coelho Lourenço Universidade de São Paulo
  • Ana Paula Almeida Ayres Universidade Estadual de Campinas
  • Taís Fonseca Mantilla Universidade de São Paulo
  • Marcelo Giannini Universidade Estadual de Campinas
  • Patricia Moreira de Freitas Universidade de São Paulo



Biomaterial S-PRG. Erosion. Union resistance.


Aim: To evaluate the bond strength of composite resin containing or not biomaterial (S-PRG) to sound/eroded dentine. Methods: Occlusal dentin of 30 human molars (n=15) had half of its surface kept uneroded, while on the other half an erosive lesion was produced by cycling in citric acid (pH 2.3) and supersaturated solution (pH 7.0). On both eroded (ED) and non-eroded (SD) substrates, two restorative systems (containing or not S-PRG) were tested. Composite resin cylinders were built and, after storage in water (24h), were submitted to bond strength test. The analysis of the fracture pattern was performed under an optical microscope (40x). The obtained values of bond strength (MPa) were submitted to ANOVA (two factors) and Tukey multiple comparisons tests (p<0.05). Results: According to the results, there was difference between substrates (<0.001) and restorative materials (p=0.002) evaluated. For the microtensile bond strength, the values obtained were: SDNB (47.6±12.2 MPa), SDWB (34.1±15.8 MPa), EDNB (31.1±8.3 MPa) and EDWB (15.5±13.6 MPa), revealing a statistically significant difference in the evaluated substrates and restorative materials. Conclusion: Bond strength of eroded substrate is inferior to the sound substrate and the restorative system containing S-PRG biomaterial influences negatively the results of bonding to sound/eroded dentin.


Download data is not yet available.


Metrics Loading ...

Author Biographies

Alessandra Sanchez Coelho Lourenço, Universidade de São Paulo

Department of Restorative Dentistry, School of Dentistry, Universidade de São Paulo (USP), SP

Ana Paula Almeida Ayres, Universidade Estadual de Campinas

Department of Dental Materials, Piracicaba Dental School, Faculdade de Odontologia de Piracicaba (UNICAMP), Piracicaba, SP

Taís Fonseca Mantilla, Universidade de São Paulo

Department of Restorative Dentistry, School of Dentistry, Universidade de São Paulo (USP), SP

Marcelo Giannini, Universidade Estadual de Campinas

Department of Dental Materials, Piracicaba Dental School, Faculdade de Odontologia de Piracicaba (UNICAMP), Piracicaba, SP.

Patricia Moreira de Freitas, Universidade de São Paulo

Special Laboratory of Lasers in Dentistry, Department of Restorative Dentistry, School of Dentistry, Universidade de São Paulo (USP), SP


Lussi A, Schlueter N, Rakhmatullina E, Ganss C. Dental Erosion - An overview with emphasis on chemical and histopathological aspects. Caries Res. 2011;45 Suppl 1:2-12.

Zero DT. Etiology of dental erosion - extrinsic factors. Eur J Oral Sei. 1996 Apr; 104(2):162- 77.

Salas MM, Nascimento GG, Vargas-Ferreira F, Tarquinio SB, Huysmans MC, Demarco FF. Diet influenced tooth erosion prevalence in children and adolescents: Results of a meta-analysis and meta-regression. J Dent. 2015 Aug;43(8):865-75.

Carvalho TS, Colon P, Ganss C, Huysmans MC, Lussi A, Schlueter N, et al. Consensus report of the European Federation of Conservative Dentistry: erosive tooth wear-diagnosis and management. Clin Oral lnvestig. 2015 Sep;19(7):1557-61.

Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munck J, et al. State of the art of self-etch adhesives. Dent Mater. 2011 Jan;27(1):17-28.

McCabe JF, Carrick TE, Kamohara H. Adhesive bond strength and compliance for denture soft lining materials. Biomaterials. 2002 Mar;23(5):1347-52.

Featherstone JDB, O’Relly MM,Shariati M, Brugler S. Enhacement of remineralization in vitro and in vivo. In: Leach SA. Factors affecting de- and remineralization of the teeth. Oxford: IRL Press; 1986. p. 23-34.

Han L, Edward C, Okamoto A, Iwaku M. A comparative study of fluoridereleasing adhesive resin materials. Dent Mat J. 2002; 21(1): 9-19.

Soares LE, Lima LR, Vieira Lde S, Do Espírito Santo AM, Martin AA. Erosion effects on chemical composition and morphology of dental materials and root dentin. Microsc Res Tech. 2012 Jun;75(6):703-10.

Gordan VV, Mondragon E, Watson RE, Garvan C, Mjor IA. A clinical evaluation of a self-etching primer and a giomer restorative material: results at eight years. J Am Dent Assoc. 2007;138(5):621-7.

Moretto SG, Azambuja N Jr, Arana-Chavez VE, Reis AF, Giannini M, Eduardo Cde P,et al.. Effects of ultramorphological changes on adhesion to lased dentin-Scanning electron microscopy and transmission electron microscopy analysis. Microsc Res Tech. 2011 Aug;74(8):720-6.

Shiozawa M, Takahashi H, Iwasaki N. Fluoride release and mechanical properties after 1-year water storage of recent restorative glass ionomer cements. Clin Oral Investig. 2014 May;18(4):1053-60.

Ayres APA, Tabchoury CP, Bittencourt Berger S, Yamauti M, Bovi Ambrosano GM, Giannini M. Effect of Fluoride-containing Restorative Materials on Dentin Adhesion and Demineralization of Hard Tissues Adjacent to Restorations. J Adhes Dent. 2015 Aug;17(4):337-45.

Ganss C, Klimek J, Schãffer U, Spall T. Effectiveness of two fluoridation measures on erosion progression in human enamel and dentin in vitro. Caries Res. 2001 Sep­Oct;35(5):325-30.

Sano H, Shono T, Sonoda H, Takatsu T, Ciucchi B, Carvalho R, et al. Relationship between surface area for adhesion and tensile bond strength-eval uation of a micro-tensile bond test. Dent Mater. 1994 Jul;10(4):236-40.

Honório HM, Rios D, Francisconi LF, MagalhÃes AC, MacHado MAAM, Buzalaf MAR. Effect of prolonged erosive pH cycling on different restorative materials. J Oral Rehabil. 2008;35(12):947-53.

Badra VV, Faraoni JJ, Ramos RP, Palma-Dibb RG. Influence of different beverages on the microhardness and surface roughness of resin composites. Oper Dent. 2005;30(2):213-9.

Wongkhantee S, Patanapiradej V, Maneenut C, Tantbirojn D. Effect of acidic food and drinks on surface hardness of enamel , dentine , and tooth-coloured filling materials. J Dent. 2006 Mar;34(3):214-20.

Pedroso C, Anderson AT, Hara T, Jorge S, Mo DÆ. Study on the potential inhibition of root dentine wear adjacent to fluoride-containing restorations. J Mater Sci Mater Med. 2008 Jan;19(1):47-51.

Amsler F, Lussi A. Long-Term Bond Strength of Self-Etch Adhesives to Normal and Artificially Eroded Dentin : Effect of Relative. 2017;19(2):169-77.

Forgerini TV Rocha R de Oliveira, Soares FZM, Lenzi TL RJF. Role of Etching Mode on Bonding Longevity of a Universal Adhesive to Eroded Dentin. J Adhes Dent. 2017;19(1):69-76.

Cruz JB, Lenzi TL, Tedesco TK, Guglielmi C de AB, Raggio DP. Eroded dentin does not jeopardize the bond strength of adhesive restorative materials. Braz Oral Res. 2012;26(4):306-12.

Wang X, Lussi A. Assessment and management of dental erosion. Dent Clin North Am. 2010;54(3):565-78.

Prati C, Montebugnoli L, Suppa P, Valdrè G, Mongiorgi R. Permeability and Morphology of Dentin after Erosion Induced by Acidic Drinks. J Periodontol. 2003;74(4):428-36.

Buzalaf MAR, Kato MT, Hannas AR. The Role of Matrix Metalloproteinases in Dental Erosion. Adv Dent Res. 2012;24(2):72-6.

Wang Y, Spencer P. Effect of acid etching time and technique on interfacial characteristics of the adhesive-dentin bond using differential staining. Eur J Oral Sci. 2004;112(3):293-9.

Masarwa N, Mohamed A, Abou-Rabii I, Abu Zaghlan R, Steier L. Longevity of Self-etch Dentin Bonding Adhesives Compared to Etch-and-rinse Dentin Bonding Adhesives: A Systematic Review. J Evid Based Dent Pract. 2016 Jun;16(2):96-106.

Giannini M, Makishi P, Ayres APA, Vermelho PM, Fronza BM, Nikaido T, et al. Self-etch adhesive systems : a literature review. Braz Dent J. 2015 Jan-Feb;26(1):3-10.

Wang Y, Spencer P. Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy. J Biomed Mater Res. 2002;59(1):46-55.

Ramos TM, Ramos-Oliveira TM, de Freitas PM, Azambuja N, Esteves-Oliveira M, Gutknecht N, et al. Effects of Er:YAG and Er,Cr:YSGG laser irradiation on the adhesion to eroded dentin. Lasers Med Sci. 2015;30(1):17-26.

Frattes FC. Bond strength to eroded enamel and dentin using a. Universal Adhesive System. J Adhes Dent. 2017;19(2):121-7.

Deari S, Wegehaupt J, Tauböck TT. Influence of different pretreatments on the microtensile bond strength to eroded dentin. J Ades Dent. 2017;19(2):147-55.

Burrow M., Nopnakeepong U, Phrukkanon S. A comparison of microtensile bond strengths of several dentin bonding systems to primary and permanent dentin. Dent Mater. 2002;18(3):239-45.

Lussi A, Carvalho TS. The future of fluorides and other protective agents in erosion prevention. Caries Res. 2015;49(suppl 1):18-29.

Paula A, Guedes A, Moda MD, Umeda TY, Gustavo A, Godas DL, et al. Effect of Fluoride-Releasing Adhesive Systems on the Mechanical Properties of Eroded Dentin. Braz Dent J. 2016 Mar-Apr;27(2):153-9.

Bollu IP, Hari A, Thumu J, Velagula LD, Bolla N, Varri S, et al. Comparative evaluation of microleakage between nano-ionomer, giomer and resin modified glass ionomer cement in class V cavities- CLSM study. J Clin Diagnostic Res. 2016;10(5):ZC66-ZC70.

Sunico MC, Shinkai K, Katoh Y. Two-year clinical performance of occlusal and cervical giomer restorations. Oper Dent. 2005;30(3):282-9.




How to Cite

Lourenço ASC, Ayres APA, Mantilla TF, Giannini M, Freitas PM de. Bond strength of composite resin containing biomaterial S-PRG to eroded dentin. Braz. J. Oral Sci. [Internet]. 2017 Dec. 15 [cited 2023 Feb. 4];16:1-10. Available from:

Most read articles by the same author(s)