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The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria


Adipose Tissue. Bone regeneration. Rabbits. Wnt proteins.

How to Cite

Klug LG, Storer CLM, Sebastiani AM, Bsrbaresco BL, Giovanini AF, Deliberador TM. The influence of the protein Wnt10b as a marker of bone repair of critical size defects fille with autogenous adipose tissue graft: A study in rabbit calvaria. Braz. J. Oral Sci. [Internet]. 2017 Dec. 15 [cited 2023 Oct. 1];16:1-11. Available from:


The proteins Wnts are considered a key regulator of the early development of the skeleton. Aim: The aim of this study was to evaluate the presence of the protein Wnt10b as a marker of bone repair in critical size defects surgically created in the calvaria of rabbits treated with fragmented autogenous adipose tissue graft. Methods: A total of 28 rabbits were divided into two groups: the Control group (C) and Adipose Tissue Graft group (ATG). A CSD measuring 15 mm in diameter was created in the calvaria of each animal. In rabbits of the C group, the defect was filled only with blood clot, and in ATG group, the defect was filled with fragmented adipose tissue graft. The two groups were divided into two subgroups (n = 7) for euthanasia 15 and 40 days after surgery. Histological and immunohistochemically analyses were performed to evaluate the neoformed bone and the presence/concentration of Wnt10b protein. The Kruskal-Wallis test was performed to compare the means and standard deviations of the number of Wnt10b + cells/mm2 in both groups in each postoperative period. It was assumed a significance level of 5%. Results: After 40 days, the mean concentration of the protein Wnt10b in ATG group was 26.26 (+-6.97) significant higher (p<0,001) than the mean in C group that was 305 (37.41). Conclusion: The protein Wnt10b would play a crucial role in the signaling of bone formation in bone defects treated with fragmented autogenous adipose tissue graft.


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