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Aim: The objective of this study was to investigate the levels of IL-10 in the gingival crevicular fluid in HIV-1 positive patients with chronic periodontitis and to compare with HIV-1 negative patients with chronic periodontitis, also to correlate clinical periodontal parameters, viral load and count of CD4+ and CD8+ lymphocytes (LTCD4+ and LTCD8+). Methods: 33 patients were selected and splitted into two groups: 16 HIV-1 positive patients and 17 HIV-1 negative patients and all with chronic periodontitis. The clinical periodontal parameters recorded were: Probing Depth (PD) and Clinical Attachment Level (CAL); the sistemical parameters LTCD4+, LTCD8+ and viral load were analized by the gingival crevicular fluid collected from all patients. Enzyme-linked immunosorbent assay (ELISA) was used to determine the concentrations of Interleukin (IL)-10. For the statistical analysis the Student t, Mann-Whitney and Spearman tests were performed. IL-10 levels were significantly lower in both patients groups. Results: There was statistical difference betwen groups for probing depth (p=0.015) and clinical attachment level (p=0.011), no significant correlation was found among the analyzed variables. Conclusion: The IL-10 levels in HIV-1 positive patients had no influence in periodontal and medical parameters.References
Rodrigues R de O, Carvalho PG, Arruda EA, Rabenhorst SH, Silva SF, Ribeiro IF, et al. Interleukin-10 gene polymorphism (-1082G/A) and allergy to efavirenz in patients infected with human immunodeficiency virus. Braz J Infect Dis. 2014 Jul-Aug;18(4):445-8. doi: 10.1016/j.bjid.2014.01.009.
Freitas FB, Lima SS, Feitosa RN, Azevedo VN, Ishak Mde O, Ishak R, et al. Polymorphisms in the IFNγ, IL-10, and TGFβ genes may be associated with HIV-1 infection. Dis Markers. 2015;2015:248571. doi: 10.1155/2015/248571.
Singh S, Sharma A, Arora SK. Combination of low producer AA-genotypes in IFN-γ and IL-10 genes makes a high risk genetic variant for HIV disease progression. Cytokine. 2016 Jan;77:135-44. doi: 10.1016/j.cyto.2015.11.009.
Jegede FE, Oyeyi TI, Abdulrahman SA, Mbah HA, Badru T, Agbakwuru C, et al. Effect of HIV and malaria parasites co-infection on immune-hematological profiles among patients attending anti-retroviral treatment (ART) clinic in Infectious Disease Hospital Kano, Nigeria. PLoS One. 2017 Mar 27;12(3):e0174233. doi: 10.1371/journal.pone.0174233.
Chin BS. Molecular Epidemiology of Human Immunodeficiency Virus. Infect Chemother. 2017 Mar;49(1):1-9. doi: 10.3947/ic.2017.49.1.1.
Noguera-Julian M, Guillén Y, Peterson J, Reznik D, Harris EV, Joseph SJ, et al. Oral microbiome in HIV-associated periodontitis. Medicine (Baltimore). 2017 Mar;96(12):e5821. doi: 10.1097/MD.0000000000005821.
Elizondo JE, Rocha-Pizaña MD, Treviño AC, Violant D, Álvarez MM, Rivas-Estilla AM. Potential gingival crevicular fluid and serum biomarkers by stage of HIV infection. Cytokine. 2017 Mar;91:96-103. doi: 10.1016/j.cyto.2016.12.019.
Bing XJ, Sun XJ, Shen GH, Xie H, Ma MY. [Levels of IL-8,IL-10 in patients with chronic periodontitis and coronary heart disease]. Shanghai Kou Qiang Yi Xue. 2015 Oct;24(5):598-601. Chinese.
Zhang Q, Chen B, Zhu D, Yan F. Biomarker levels in gingival crevicular fluid of subjects with different periodontalconditions: A cross-sectional study. Arch Oral Biol. 2016 Dec;72:92-98. doi: 10.1016/j.archoralbio.2016.08.020.
Guimarães G, Franco GCN, Cortelli JR, Cogo K, Costa FO, Aquino DR, et al. [Subjects harboring HIV show poor clinical periodontal condition and higher prevalence of Porphyromonas gingivalis and Tannerella forsythia]. Rev Odontol UNESP. 2012 Jan-Feb;41(1):15-21. [Portuguese].
Jiang C, Liu S, Liu S, Li Z, Chen P, Chen L. Association Between the Interleukin-10-1082G/A, -592C/A, -819C/T Gene Polymorphism and HIV-1 Susceptibility: A Meta-Analysis. AIDS Res Hum Retroviruses. 2017 Jan;33(1):61-7. doi: 10.1089/AID.2016.0056.
Goutoudi P, Diza E, Arvanitidou M. Effect of periodontal therapy on crevicular fluid interleukin-6 and interleukin-8 levels in chronic periodontitis. Int J Dent. 2012;2012:362905. doi: 10.1155/2012/362905.
Severino VO, Beghini M, de Araújo MF, de Melo ML, Miguel CB, Rodrigues WF, et al. Expression of IL-6, IL-10, IL-17 and IL-33 in the peri-implant crevicular fluid of patients with peri-implant mucositis and peri-implantitis. Arch Oral Biol. 2016 Dec;72:194-9. doi: 10.1016/j.archoralbio.2016.08.021.
Pirim Gorgun E, Toker H, Korkmaz EM, Poyraz O. IL-6 and IL-10 gene polymorphisms in patients with aggressive periodontitis: effects on GCF, serum and clinic parameters. Jan 16;31:e12. doi: 10.1590/1807-3107BOR-2017.vol31.0012.
Passoja A, Puijola I, Knuuttila M, Niemelä O, Karttunen R, Raunio T, et al. Serum levels of interleukin-10 and tumour necrosis factor-α in chronic periodontitis. J Clin Periodontol. 2010 Oct;37(10):881-7. doi: 10.1111/j.1600-051X.2010.01602.x.
Silveira VR, Pigossi SC, Scarel-Caminaga RM, Cirelli JA, Rêgo R, Nogueira NA. Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis. Braz Oral Res. 2016 Oct 10;30(1):e105. doi: 10.1590/1807-3107BOR-2016.vol30.0105.
Moretti S, Bartolommei L, Galosi C, Renga G, Oikonomou V, Zamparini F, et al. Fine-tuning of Th17 Cytokines in Periodontal Disease by IL-10. J Dent Res. 2015 Sep;94(9):1267-75. doi: 10.1177/0022034515591790.
John CN, Stephen LX , Joyce África CW. Is human immunodeficiency virus (HIV) stage an independent risk factor for altering the periodontal status of HIV-positive patients? A South African study. BMC Saúde Oral 2013 Dez 3;13:69. doi: 10.1186/1472-6831-13-69.
Dmello DM, Ariyanto I, Estiasari R, Halstrom S, Gaff J, Lee S, et al. Polymorphisms in IL10 may alter CD4 T-cell counts in Indonesian HIV patients beginning antiretroviral therapy. Hum Immunol. 2017 Apr;78(4):387-90. doi: 10.1016/j.humimm.2017.03.001.
Ujiie Y, Karakida T, Yamakoshi Y, Ohshima T, Gomi K, Oida S. Interleukin-4 released from human gingival fibroblasts reduces osteoclastogenesis. Arch Oral Biol. 2016 Dec;72:187-93. doi: 10.1016/j.archoralbio.2016.08.024.
Jaradat SM, Ababneh KT, Jaradat SA, Abbadi MS, Taha AH, Karasneh JA, et al. Association of interleukin-10 gene promoter polymorphisms with chronic and aggressive periodontitis. Oral Dis. 2012;18(3):271-9. doi: 10.1111/j.1601-0825.2011.01872.x.
Teles RP, Likhari V, Socransky SS, Haffajee AD. Salivary cytokine levels in subjects with chronic periodontitis and in periodontallyhealthy individuals: a cross-sectional study. J Periodontal Res. 2009 Jun;44(3):411-7. doi: 10.1111/j.1600-0765.2008.01119.x.
Cullinan MP, Westerman B, Hamlet SM, Palmer JE, Faddy MJ, Seymour GJ et al. Progression of periodontal disease and interleukin-10 gene polymorphism. J Periodontal Res. 2008 Jun;43(3):328-33. doi: 10.1111/j.1600-0765.2007.01034.x.
Lappin DF, MacLeod CP, Kerr A, Mitchell T, Kinane DF. Anti-inflammatory cytokine IL-10 and T cell cytokine profile in periodontitis granulation tissue. Clin Exp Immunol. 2001;123(2):294-300.
Noro Filho GA, Salgado DM, Casarin RC, Casati MZ, Costa C, Giovani EM. Anti-infective periodontal therapy promoting improvement in systemic markers of HIV infection. AIDS Res Hum Retroviruses. 2013 Jul;29(7):1040-4. doi: 10.1089/AID.2012.0359.
Armitage GC. Periodontal diseases: diagnosis. Ann Periodontol. 1996 Nov;1(1):37-215.
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