Effect of chemical and natural irrigant solutions on microhardness of root dentin – an in vitro study

Authors

  • Caren Taffarel University of Passo Fundo
  • Fernanda Duda Bonatto University of Passo Fundo
  • Filipe do Carmo Bonfante University of Passo Fundo
  • Huriel Scartazzini Palhano University of Passo Fundo
  • Cristina de Mattos Pimenta Vidal University of Iowa
  • Doglas Cecchin, Matheus Albino Souza University of Passo Fundo
  • Matheus Albino Souza University of Passo Fundo

DOI:

https://doi.org/10.20396/bjos.v17i0.8654060

Keywords:

Calcium hypochlorite. Chlorhexidine. Grape seed extract. Sodium hypochlorite. Hardness.

Abstract

Introduction: Modifications in the mechanical properties of dentin may reduce the fracture resistance of tooth, especially after endodontic treatment. The aim of present study was to evaluate the effect of the irrigation with different root canal irrigants on the microhardness of root dentin. Methods: The coronal portion of 60 single-rooted bovine incisors was sectioned and the pulpal tissue removed using endodontic K-files. The roots were cut transversely to obtain 2 fragments, which were embedded in acrylic resin and randomly distributed into six groups (n=20) according to the irrigation protocol: distilled water (DW) (control); 2% chlorhexidine solution (CHX); 6% sodium hypochlorite (NaOCl); 6% calcium hypochlorite (Ca[OCl]2); QMix; and 6.5% grape seed extract solution (GSE). The solutions were kept in contact with the root dentin specimens for 30 min. After that, irrigation with 5 mL of DW was performed. The Vickers microhardness was determined by performing three indentations in all specimens, using 300-g load and 20-second dwell time. The first indentation was made 1.000 µm from the root canal entrance, and two other indentations were made at a distance of 200 µm from each other. The microhardness value for each specimen was obtained as the average of the results for the three indentations. Data were statistically analyzed using one-way ANOVA with 5% significance level. Results: All the tested irrigant solutions maintained the same microhardness level of the root dentin when compared to the control group, with no statistically significant differences between them (p<0.05) Conclusion: The tested irrigant solutions did not present ability to modify the microhardness of root dentin.

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Author Biographies

Caren Taffarel, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

Fernanda Duda Bonatto, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

Filipe do Carmo Bonfante, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

Huriel Scartazzini Palhano, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

Cristina de Mattos Pimenta Vidal, University of Iowa

College of Dentistry and Dental Clinics, University of Iowa.

Doglas Cecchin, Matheus Albino Souza, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

Matheus Albino Souza, University of Passo Fundo

School of Dentistry, University of Passo Fundo.

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Published

2018-11-26

How to Cite

1.
Taffarel C, Bonatto FD, Bonfante F do C, Palhano HS, Vidal C de MP, Souza DCMA, Souza MA. Effect of chemical and natural irrigant solutions on microhardness of root dentin – an in vitro study. Braz. J. Oral Sci. [Internet]. 2018 Nov. 26 [cited 2022 Aug. 8];17:e18409. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8654060

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