Banner Portal
Long-term evaluation of crown and root discoloration induced by different endodontic sealers


Endodontics. Root canal filling materials. Tooth crown. Tooth discoloration.

How to Cite

Coelho BS, Garcia PP, Tomazinho FSF, Cunha LF, Leonardi DP, Gonzaga CC, Gabardo MCL, Baratto-Filho F. Long-term evaluation of crown and root discoloration induced by different endodontic sealers. Braz. J. Oral Sci. [Internet]. 2018 Dec. 4 [cited 2023 Dec. 10];17:e18483. Available from:


Aim: To evaluate crown and root discoloration promoted by different endodontic sealers after root canal filling. Methods: Eighty bovine incisors were prepared and filled with: Endofill, Sealer 26, AH Plus, and MTA Fillapex. Color was recorded using a spectrophotometer before endodontic treatment (T0) and at 24 hours (T1), seven days (T2), 30 days (T3), and 90 days (T4) after treatment. Analyses were performed on the middle and cervical regions of the crown, and on the cervical third of the root, immediately below the cementoenamel junction. The color alterations (ΔE) were calculated using Commission International de I’Eclairage (CIE) L*a*b parameters, and data were analyzed by analysis of variance (ANOVA) and Tukey’s test (p<0.05). Results: All sealers induced color alterations. Sealer 26 resulted in the smallest changes in color (E = 5.32). The other materials did not present statistical differences (AH Plus E = 6.98; MTA Fillapex E = 6.88; Endofill E = 6.41). Of the three regions analyzed, the largest discoloration was observed at the cervical third of the root (E=10.67). In terms of time, the largest ΔE values (E=7.72) were observed at T4. Color changes at T1 (E=5.88), T2 (E=6.10), and T3 (E=5.89) were statistically similar. Conclusions: All endodontic sealers promoted discoloration on the tooth crown and root.


Ahmed HMA, Abbott PV. Discolouration potential of endodontic procedures and materials: a review. Int Endod J. 2012 Oct;45(10):883-97. doi: 10.1111/j.1365-2591.2012.02071.x.

Zare Jahromi M, Navabi AA, Ekhtiari M. Comparing coronal discoloration between AH26 and ZOE sealers. Iran Endod J. 2011 Fall;6(4):146-9.

Lenherr P, Allgayer N, Weiger R, Filippi A, Attin T, Krastl G. Tooth discoloration induced by endodontic materials: a laboratory study. Int Endod J. 2012 Oct;45(10):942-9. doi: 10.1111/j.1365-2591.2012.02053.x.

Meincke DK, Prado M, Gomes BPF, Bona AD, Sousa ELR. Effect of endodontic sealers on tooth color. J Dent. 2013 Aug;41(Suppl 3):e93-6.

Ioannidis K, Mistakidis I, Beltes P, Karagiannis V. Spectrophotometric analysis of crown discoloration induced by MTA- and ZnOE-based sealers. J Appl Oral Sci. 2013 Mar-Apr;21(2):138-44. doi: 10.1590/1678-7757201302254.

Forghani M, Gharechahi M, Karimpour S. In vitro evaluation of tooth discolouration induced by mineral trioxide aggregate Fillapex and iRoot SP endodontic sealers. Aust Endod J. 2016 Dec;42(3):99-103. doi: 10.1111/aej.12144.

Parsons JR, Walton RE, Ricks-Williamson L. In vitro longitudinal assessment of coronal discoloration from endodontic sealers. J Endod. 2001 Nov;27(11):699-702.

Partovi M, Al-Havvaz AH, Soleimani B. In vitro computer analysis of crown discolouration from commonly used endodontic sealers. Aust Endod J. 2006 Dec;32(3):116-9.

Walsh LJ, Athanassiadis B. Endodontic aesthetic iatrodontics. Aust Dental Prac. 2007;18:62-4.

Allan NA, Walton RC, Schaeffer MA. Setting times for endodontic sealers under clinical usage and in vitro conditions. J Endod. 2001 Jun;27(6):421-3.

Davis MC, Walton RE, Rivera EM. Sealer distribution in coronal dentin. J Endod. 2002 Jun;28(6):464-6.

Asgary S, Parirokh M, Eghbal MJ, Brink F. Chemical differences between white and gray mineral trioxide aggregate. J Endod. 2005 Feb;31(2):101-3.

Ioannidis K, Beltes P, Lambrianidis T, Kapagiannidis D, Karagiannis V. Validation and spectrophotometric analysis of crown discoloration induced by root canal sealers. Clin Oral Investig. 2013 Jul;17(6):1525-33. doi: 10.1007/s00784-012-0850-x.

Kohli MR, Yamaguchi M, Setzer FC, Karabucak B. Spectrophotometric analysis of coronal tooth discoloration induced by various bioceramic cements and other endodontic materials. J Endod. 2015 Nov;41(11):1862-6. doi: 10.1016/j.joen.2015.07.003.

Shokouhinejad N, Nekoofar MH, Pirmoazen S, Shamshiri AR, Dummer PM. Evaluation and comparison of occurrence of tooth discoloration after the application of various calcium silicate-based cements: an ex vivo study. J Endod. 2016 Jan;42(1):140-4. doi: 10.1016/j.joen.2015.08.034.

International Commission on Illumination. Recommendations on uniform color spaces, color-difference equations, psychometric color terms. Bureau Central de la CIE. 1978;15:13.

Johnston WM, Kao EC. Assessment of appearance match by visual observation and clinical colorimetry. J Dent Res. 1989 May;68(5):819-22.

Thomson AD, Athanassiadis B, Kahler B, Walsh L. Tooth discolouration: staining effects of various sealers and medicaments. Aust Endod J. 2012 Apr;38(1):2-9. doi: 10.1111/j.1747-4477.2011.00339.x.

Costa BM, Iwamoto AS, Puppin-Rontani RM, Pascon FM. Comparative analysis of root dentin morphology and structure of human versus bovine primary teeth. Microsc Microanal. 2015 Jun;21(3):689-94. doi: 10.1017/S1431927615000434.

Lisbôa EI, Silva Neto UX da, Carneiro E, Fariniuk LF, Westphalen VPD. Capacidade de selamento e penetração intratubular do MTA Fillapex e do AH Plus em dentes humanos. Rev Odontol UNESP. 2017;46(1):7-13. doi: 10.1590/1807-2577.25915.

Gürel MA, Kivanç BH, Ekici A, Alaçam T. Evaluation of crown discoloration induced by endodontic sealers and change ratio determination after bleaching. Aust Endod J. 2016 Dec;42(3):119-123. doi: 10.1111/aej.12147.

Suciu I, Ionescu E, Dimitriu BA, Bartok RI, Moldoveanu GF, Gheorghiu IM, et al. An optical investigation of dentinal discoloration due to commonly endodontic sealers, using the transmitted light polarizing microscopy and spectrophotometry. Rom J Morphol Embryol. 2016;57(1):153-9.

Yassen GH, Platt JA, Hara AT. Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci. 2011 Sep;53(3):273-82.

The Brazilian Journal of Oral Sciences uses the Creative Commons license (CC), thus preserving the integrity of the articles in an open access environment.


Download data is not yet available.


Metrics Loading ...