Effect of cavity-cleaning agents on shear bond strength between self-etching adhesives and dentin

  • Tamiris Alacoque de Carvalho Freitas School of Dentistry, Federal University of Juiz de Fora
  • Bruno Salles Sotto-Maior School of Dentistry, Federal University of Juiz de Fora
  • Mariele Ferraz de Oliveira School of Dentistry, Federal University of Juiz de Fora
  • Fabiana Cesar Costa School of Dentistry, Federal University of Juiz de Fora
  • Luciana Andrea Salvio School of Dentistry, Federal University of Juiz de Fora
Keywords: Chlorhexidine, Dental bonding, Adhesives.

Abstract

Aim: The effect of cavity-cleaning agents on shear bonding strength between self-etching adhesives and dentin was evaluated. Methods: Twenty-five healthy human third molars were worn to obtain flat middle dentin. The samples were randomly divided into five groups according to the cleaning agent used: group CB not treated with any cavity disinfectants and served as control. Following groups, dentin surfaces were treated with 2% chlorhexidine (CHX group); 3% hydrogen peroxide (HP group); calcium hydroxide water (CH group); and 70% ethyl ethanol (E group). The dentin surfaces were cleaned by friction for 20 s and dentine bonding agent, Clearfil SE Bond, was applied. Cylinders (5.0 mm  1.2 mm, n = 19) of fluid composite resin were placed on the dentin surface, and the teeth were stored in deionized water at 37°C for 24 h. Subsequently, the samples were subjected to the shear bonding test, and the obtained values were subjected to one-way ANOVA and Tukey’s test (α = 0.05). The failure pattern was analyzed descriptively by examining the specimens with a stereoscope at a magnification of 4, and failure was classified into adhesive, cohesive or mixed type. Results: Shear bonding was significantly lower in group HP (6.17 ± 5.69 MPa). The groups CB, CHX, CH and E were statiscally similar, 22.15 ± 6.54MPa; 18.40 ± 7.26MPa; 18.07 ± 5.98MPa ; 16.43 ± 6.19MPa respectively. The failure modes observed were only adhesive and mixed. Conclusion: The use of 2% chlorhexidine, calcium hydroxide water, and 70% ethyl ethanol did not negatively affect bond strength. Only 3% hydrogen peroxide is contraindicated as a cavity cleaner in adhesive restorative procedures when self-etching system is used.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Tamiris Alacoque de Carvalho Freitas, School of Dentistry, Federal University of Juiz de Fora
Department of Restorative Dentistry
Bruno Salles Sotto-Maior, School of Dentistry, Federal University of Juiz de Fora
Department of Restorative Dentistry
Mariele Ferraz de Oliveira, School of Dentistry, Federal University of Juiz de Fora
Department of Restorative Dentistry
Fabiana Cesar Costa, School of Dentistry, Federal University of Juiz de Fora
Department of Restorative Dentistry
Luciana Andrea Salvio, School of Dentistry, Federal University of Juiz de Fora
Department of Restorative Dentistry

References

Elkassas DW, Fawzi EM, Zohairy AE. The effect of cavity disinfectants on the micro-shear bond strength of dentin adhesives. Eur J Dent. 2014 Apr;8(2):184-90. doi: 10.4103/1305-7456.130596.

Dionysopoulos D. Effect of digluconate chlorhexidine on bond strength between dental adhesive systems and dentin: A systematic review. J Conserv Dent. 2016 Jan-Feb;19(1):11-6. doi: 10.4103/0972-0707.173185.

Say EC, Koray F, Tarim B, Soyman M, Gulmez T. In vitro effect of cavity disinfectants on the bond strength of dentin bonding systems. Quintessence Int. 2004 Jan;35(1):56-60.

Shinohara MS, Bedran-de-Castro AKB, Amaral CM, Pimenta LAF. The effect of sodium hypochlorite on microleakage of composite resin restorations using three adhesive systems. J Adhes Dent. 2004 Aug;6(2):123-7.

Ercan E, Erdemir A, Zorba OY, Eldeniz AU, Dalli M, Ínce B, Kalaycioglu B. Effect of different cavity disinfectants on shear bond strength of composite resin to dentin. J Adhes Dent. 2009 Oct;11(5):343-6.

Sharma V, Rampal P, Kumar S. Shear bond strength of composite resin to dentin after application of cavity disinfectants – SEM study. Contemp Clin Dent. 2011 Jul;2(3):155-9. doi: 10.4103/0976-237X.86438.

Tulunoglu O, Ayhan H, Olmez A, Bodur H. The effect of cavity disinfectants on microleakage in dentin bonding systems. J Clin Pediatr Dent. 1998 Summer;22(4):299-305.

Soares CJ, Pereira CA, Pereira JC, Santana FR, do Prado CJ. Effect of chlorhexidine application on microtensile bond strength to dentin. Oper Dent. 2008 Mar-Apr;33(2):183-8. doi: 10.2341/07-69.

Pappas M, Burns DR, Moon PC, Coffey JP. Influence of a 3-step tooth disinfection procedure on dentin bond strength. J Prosthet Dent. 2005 Jun;93(6):545-50.

Frassetto A, Breschi L, Turco G, Marchesi G, Lenarda RD, Tay FR, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability- A literature review. Dent Mater. 2016 Feb;32(2):e41-53. doi: 10.1016/j.dental.2015.11.007.

Cha HS, Shin DH. Antibacterial capacity of cavity disinfectants against Streptococcus mutans and their effects on shear bond strength of a self-etch adhesive. Dent Mater J. 2016;35(1):147-52. doi: 10.4012/dmj.2015-175.

Hashimoto M, Ohno H, Sano H, Kaga M, Oguchi H. In vitro degradation of resin–dentin bonds analyzed by microtensile bond test, scanning and transmission electron microscopy. Biomaterials. 2003 Sep;24(21):3795-803.

Kermanshahi S, Santerre JP, Cvitkovitch DG, Finer Y. Biodegradation of resin-dentin interfaces increases bacterial microleakage. J Dent Res. 2010 Sep;89(9):996-1001. doi: 10.1177/0022034510372885.

Spencer P, Wang Y. Adhesive phase separation at the dentin interface under wet bonding conditions. J Biomed Mater Res. 2002 Dec 5;62(3):447-56.

Ye Q, Park J, Topp E, Spencer P. Effect of photoinitiators on the in vitro performance of a dentin adhesive exposed to simulated oral environment. Dent Mater. 2009 Apr;25(4):452-8. doi: 10.1016/j.dental.2008.09.011.

Wang Y, Spencer P. Quantifying adhesive penetration in adhesive/dentin interface using confocal Raman microspectroscopy. J Biomed Mater Res. 2002 Jan;59(1):46-55.

Pashley DH, Tay FR, Yiu C, Hashimoto M, Breschi L, Carvalho RM. Collagen degradation by host-derived enzymes during aging. J Dent Res. 2004 Mar;83(3):216-21.

Mazzoni A, Pashley DH, Nishitani Y, Breschi L, Mannello F, Tjäderhane L, et al. Reactivation of inactivated endogenous proteolytic activities in phosphoric acid-etched dentine by etch-and-rinse adhesives. Biomaterials. 2006 Sep;27(25):4470-6.

Hashimoto M, Hirose N, Kitagawa H, Yamaguchi S, Imazato S. Improving the durability of resin-dentin bonds with na antibacterial monomer MDPB. Dent Mater J. 2018 Jul 29;37(4):620-627. doi: 10.4012/dmj.2017-209.

Scaffa PMC, Vidal CMP, Barros N, Gesteira TF, Carmona AK, Breschi L, et al. Chlorhexidine inhibits the activity of dental cysteine cathepsins. J Dent Res. 2012 Apr;91(4):420-5. doi: 10.1177/0022034511435329.

Carrilho MR, Carvalho RM, de Goes MF, di Hipólito V, Geraldeli S, Tay FR, et al. Chlorhexidine preserves dentin bond in vitro. J Dent Res. 2007 Jan;86(1):90-4.

Ricci HA, Sanabe ME, de Souza Costa CA, Pashley DH, Hebling J. Chlorhexidine increases the longevity of in vivo resin dentin bonds. Eur J Oral Sci. 2010 Aug;118(4):411-6. doi: 10.1111/j.1600-0722.2010.00754.x.

Stanislavczuk R, Reis A, Loguercio AD. A 2 year in vitro evaluation of a chlorhexidine containing acid on the durability of resin dentin interfaces. J Dent. 2011 Jan;39(1):40-7. doi: 10.1016/j.jdent.2010.10.001.

Mohammed Hassan AM, Ali Goda A, Baroudi K. The effect of different disinfecting agents on bond strength of resin composites. Int J Dent. 2014;2014:231235. doi: 10.1155/2014/231235.

Di Hipólito V, Rodrigues FR, Piveta FB, Azevedo LC, Alonso RCB, Silikas N, et al. Effectiveness of self-adhesive luting cements in bonding to chlorhexidine-treated dentin. Dent Mater. 2012 May;28(5):495-501. doi: 10.1016/j.dental.2011.11.027.

Carrilho MR, Carvalho RM, Sousa EN, Nicolau J, Breschi L, Mazzoni A, et al. Substantivity of chlorhexidine to human dentin. Dent Mater. 2010 Aug;26(8):779-85. doi: 10.1016/j.dental.2010.04.002.

Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munk J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater. 2011 Jan;27(1):17-28. doi: 10.1016/j.dental.2010.10.023.

Varoni E, Tarce M, Lodi G, Carrassi A. Chlorhexidine (CHX) in dentistry: state of the art. Minerva Stomatol. 2012 Sep;61(9):399-419.

Zhou J, Tan J, Yang X, Xu X, Li D, Chen L. MMP-inhibitory effect of chlorhexidine applied in a self-etching adhesive. J Adhes Dent. 2011 Apr;13(2):111-5. doi: 10.3290/j.jad.a18783.

Moszner N, Salz U, Zimmermann J. Chemical aspects of self-etching enamel-dentin adhesives: a systematic review. Dent Mater. 2005 Oct;21(10):895-910.

Maciel KT, Carvalho RM, Ringle RD, Preston CD, Russell CM, Pashley DH. The effects of acetone, ethanol, HEMA, air on the stiffness of human decalcified dentin matrix. J Den Res. 1996 Nov;75(11):1851-8.

Morrison RT, Boyd RN. Organic chemistry. 3th ed. Boston: Allyn and Bacon; 1973.

Published
2019-04-08
How to Cite
Freitas, T. A. de C., Sotto-Maior, B. S., Oliveira, M. F. de, Costa, F. C., & Salvio, L. A. (2019). Effect of cavity-cleaning agents on shear bond strength between self-etching adhesives and dentin. Brazilian Journal of Oral Sciences, 18, e190886. https://doi.org/10.20396/bjos.v18i0.8655149