Keywords
Mast cells
Infection
Inflammation
Humans
Mouth mucosa.
Infection
Inflammation
Humans
Mouth mucosa.
How to Cite
1.
Souto GR, Jesus AO de, Kato Segundo T, Perdigão PF, Costa F de O, Mesquita RA de. Mast cell degranulation in periodontal disease from HIV-infected individuals. Braz. J. Oral Sci. [Internet]. 2019 Sep. 10 [cited 2024 Jul. 1];18:e191445. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8656600
Abstract
Aim: The objective was to compare the density and degranulation of mast cells on specimens obtained from individuals diagnosed with gingivitis or chronic periodontitis who were either non-HIV-infected or HIV-infected patients treated with highly active antiretroviral therapy (HAART). Methods: Gingival samples were taken from 16 non-HIV-infected individuals and 17 HIV-infected individuals diagnosed with gingivitis and chronic periodontitis. The samples were processed and stained with 0.3 percent o-toluidine blue. Densities (cells/mm²) and percentages of intact and degranulated mast cells were obtained. Results: No statistically significant differences were observed in the mast cell density and the percentage of degranulated mast cells between non-HIV-infected and HIV-infected individuals diagnosed with gingivitis and chronic periodontitis. Mononuclear inflammatory infiltrate was weakly correlated with the percentage of mast cells degranulated for both groups. Conclusions: There are no differences of the density and degranulation of mast cells in gingival tissue between non-HIV-infected and HIV-infected patients undergoing HAART, both groups with diagnosis of gingivitis or chronic periodontitis. This may be a result of the recovery of the immunologic system by HAART treatment.
References
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9. Welle M. Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase. J Leukoc Biol. 1997 Mar;61(3):233-45.
10. Villa I, Skokos D, Tkaczyk C, Peronet R, David B, Huerre M, et al. Capacity of mouse mast cells to prime T cells and to induce specific antibody responses in vivo Immunology. 2001 Feb;102(2):165-72.
11. Steinsvoll S, Helgeland K, Schenck K. Mast cells--a role in periodontal diseases? J Clin Periodontol. 2004 Jun;31(6):413-9.
12. Forsythe P, Befus AD. Inhibition of calpain is a component of nitric oxide-induced down-regulation of human mast cell adhesion. J Immunol. 2003 Jan 1;170(1):287-93.
13. Schwartz LB, Austen KF. Enzymes of the mast cell granule. J Invest Dermatol. 1980 May;74(5):349-53.
14. Marshall JS, King CA, McCurdy JD. Mast cell cytokine and chemokine responses to bacterial and viral infection. Curr Pharm Des. 2003;9(1):11-24.
15. Myint M, Steinsvoll S, Yuan ZN, Johne B, Helgeland K, Schenck K. Highly increased numbers of leukocytes in inflamed gingiva from patients with HIV infection. AIDS. 2002 Jan 25;16(2):235-43.
16. Naesse EP, Schreurs O, Helgeland K, Schenck K, Steinsvoll S. Matrix metalloproteinases and their inhibitors in gingival mast cells in persons with and without human immunodeficiency virus infection. J Periodontal Res. 2003 Dec;38(6):575-82.
17. Segundo TK, Souto GR, Costa FO, Mesquita RA. Mast cells in periodontal disease of individuals with and without HIV undergoing highly active antiretroviral therapy. J Periodontol. 2013 Jul;84(7):995-1001. doi: 10.1902/jop.2012.120436.
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19. López NJ, Smith PC, Gutierrez J. Periodontal therapy may reduce the risk of preterm low birth weight in women with periodontal disease: a randomized controlled trial. J Periodontol. 2002 Aug;73(8):911-24.
20. López NJ, Da Silva I, Ipinza J, Gutiérrez J. Periodontal therapy reduces the rate of preterm low birth weight in women with pregnancy-associated gingivitis. J Periodontol. 2005 Nov;76 Suppl 11S:2144-2153. doi: 10.1902/jop.2005.76.11-S.2144.
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22. Cohen J. Statistical power analysis for the behavioral sciences. 2nd ed. Hillsdale, NJ: Lawrence Erlbaum Associates; 1988. p. 40-151.
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24. Crowe S, Zhu T, Muller WA. The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection. J Leukoc Biol. 2003 Nov;74(5):635-41.
25. Sundstrom JB, Little DM, Villinger F, Ellis JE, Ansari AA. Signaling through Toll-like receptors triggers HIV-1 replication in latently infected mast cells. J Immunol. 2004 Apr 1;172(7):4391-401.
26. Maurer M, Theoharides T, Granstein RD, Bischoff SC, Bienenstock J, Henz B, et al. What is the physiological function of mast cells? Exp Dermatol. 2003 Dec;12(6):886-910.
27. Caughey GH. Mast cell tryptases and chymases in inflammation and host defense. Immunol Rev. 2007 Jun;217:141-54.
28. Souto GR, Queiroz-Junior CM, de Abreu MH, Costa FO, Mesquita RA. Pro-inflammatory, Th1, Th2, Th17 cytokines and dendritic cells: a cross-sectional study in chronic periodontitis. PLoS One. 2014 Mar 26;9(3):e91636. doi: 10.1371/journal.pone.0091636. eCollection 2014.
29. Lamster IB, Grbic JT, Bucklan RS, Mitchell-Lewis D, Reynolds HS, Zambon JJ. Epidemiology and diagnosis of HIV-associated periodontal diseases. Oral Dis. 1997 May;3 Suppl 1:S141-8.
30. Gonçalves LS, Gonçalves BM, Fontes TV. Periodontal disease in HIV-infected adults in the HAART era: Clinical, immunological, and microbiological aspects. Arch Oral Biol. 2013 Oct;58(10):1385-96. doi: 10.1016/j.archoralbio.2013.05.002.
31. Mataftsi M, Skoura L, Sakellari D. HIV infection and periodontal diseases: an overview of the post-HAART era. Oral Dis. 2011 Jan;17(1):13-25. doi: 10.1111/j.1601-0825.2010.01727.x.
32. Ryder MI, Nittayananta W, Coogan M, Greenspan D, Greenspan JS. Periodontal disease in HIV/AIDS. Periodontol 2000. 2012 Oct;60(1):78-97. doi: 10.1111/j.1600-0757.2012.00445.x.
33. Peppes CP, Lemos ASP, Araujo RLF, Portugal MEG, Buffon MCM, Raboni SM. Oral lesions frequency in HIV-positive patients at a tertiary hospital, Southern Brazil. Bra J of Oral Sci. 2013;12(3):216-22. doi: 10.1590/S1677-32252013000300012.
34. Groenewegen H, Bierman WFW, Delli K, Dijkstra PU, Nesse W, Vissink A, et al. Severe periodontitis is more common in HIV- infected patients. J Infect. 2019 Mar;78(3):171-177. doi: 10.1016/j.jinf.2018.11.008.
35. Jiang AP, Jiang JF, Wei JF, Guo MG, Qin Y, Guo QQ, et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol. 2015 Dec 30;90(6):2928-37. doi: 10.1128/JVI.03008-15.
2. Batista AC, Rodini CO, Lara VS. Quantification of mast cells in different stages of human periodontal disease. Oral Dis. 2005 Jul;11(4):249-54.
3. Juneja M, Mahajan S, Rao NN, George T, Boaz K. Histochemical analysis of pathological alterations in oral lichen planus and oral lichenoid lesions. J Oral Sci. 2006 Dec;48(4):185-93.
4. Rao KN, Brown MA. Mast cells: multifaceted immune cells with diverse roles in health and disease. Ann N Y Acad Sci. 2008 Nov;1143:83-104. doi: 10.1196/annals.1443.023.
5. Sharma R, Sircar K, Singh S, Rastogi V. Role of mast cells in pathogenesis of oral lichen planus. J Oral Maxillofac Pathol. 2011 Sep;15(3):267-71. doi: 10.4103/0973-029X.86674.
6. Ghalayani P, Jahanshahi G, Saberi Z. Degranulated mast cells and TNF-α in oral lichen planus and oral lichenoid reactions diseases. Adv Biomed Res. 2012;1:52. doi: 10.4103/2277-9175.100161.
7. Huang S, Lu F, Chen Y, Huang B, Liu M. Mast cell degranulation in human periodontitis. J Periodontol. 2013 Feb;84(2):248-55. doi: 10.1902/jop.2012.120066.
8. Guimarães JV, Costa FB, Andrade WM, Vêncio EF, Salge AK, Siqueira KM, et al. Quantification of mast cells in the uterine cervix of women infected with human immunodeficiency virus. Ann Diagn Pathol. 2011 Oct;15(5):318-22. doi: 10.1016/j.anndiagpath.2011.02.009.
9. Welle M. Development, significance, and heterogeneity of mast cells with particular regard to the mast cell-specific proteases chymase and tryptase. J Leukoc Biol. 1997 Mar;61(3):233-45.
10. Villa I, Skokos D, Tkaczyk C, Peronet R, David B, Huerre M, et al. Capacity of mouse mast cells to prime T cells and to induce specific antibody responses in vivo Immunology. 2001 Feb;102(2):165-72.
11. Steinsvoll S, Helgeland K, Schenck K. Mast cells--a role in periodontal diseases? J Clin Periodontol. 2004 Jun;31(6):413-9.
12. Forsythe P, Befus AD. Inhibition of calpain is a component of nitric oxide-induced down-regulation of human mast cell adhesion. J Immunol. 2003 Jan 1;170(1):287-93.
13. Schwartz LB, Austen KF. Enzymes of the mast cell granule. J Invest Dermatol. 1980 May;74(5):349-53.
14. Marshall JS, King CA, McCurdy JD. Mast cell cytokine and chemokine responses to bacterial and viral infection. Curr Pharm Des. 2003;9(1):11-24.
15. Myint M, Steinsvoll S, Yuan ZN, Johne B, Helgeland K, Schenck K. Highly increased numbers of leukocytes in inflamed gingiva from patients with HIV infection. AIDS. 2002 Jan 25;16(2):235-43.
16. Naesse EP, Schreurs O, Helgeland K, Schenck K, Steinsvoll S. Matrix metalloproteinases and their inhibitors in gingival mast cells in persons with and without human immunodeficiency virus infection. J Periodontal Res. 2003 Dec;38(6):575-82.
17. Segundo TK, Souto GR, Costa FO, Mesquita RA. Mast cells in periodontal disease of individuals with and without HIV undergoing highly active antiretroviral therapy. J Periodontol. 2013 Jul;84(7):995-1001. doi: 10.1902/jop.2012.120436.
18. Armitage GC. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004;34:9-21.
19. López NJ, Smith PC, Gutierrez J. Periodontal therapy may reduce the risk of preterm low birth weight in women with periodontal disease: a randomized controlled trial. J Periodontol. 2002 Aug;73(8):911-24.
20. López NJ, Da Silva I, Ipinza J, Gutiérrez J. Periodontal therapy reduces the rate of preterm low birth weight in women with pregnancy-associated gingivitis. J Periodontol. 2005 Nov;76 Suppl 11S:2144-2153. doi: 10.1902/jop.2005.76.11-S.2144.
21. Salvi GE, Lindhe J, Lang NP. Plano de tratamento de pacientes com doenças periodontais. 5th ed. Rio de Janeiro: Guanabara Koogan; 2008.p. 629.
22. Cohen J. Statistical power analysis for the behavioral sciences. 2nd ed. Hillsdale, NJ: Lawrence Erlbaum Associates; 1988. p. 40-151.
23. Bressenot A, Salleron J, Bastien C, Danese S, Boulagnon-Rombi C, Peyrin-Biroulet L. Comparing histological activity indexes in UC. Gut. 2015 Sep;64(9):1412-8. doi: 10.1136/gutjnl-2014-307477.
24. Crowe S, Zhu T, Muller WA. The contribution of monocyte infection and trafficking to viral persistence, and maintenance of the viral reservoir in HIV infection. J Leukoc Biol. 2003 Nov;74(5):635-41.
25. Sundstrom JB, Little DM, Villinger F, Ellis JE, Ansari AA. Signaling through Toll-like receptors triggers HIV-1 replication in latently infected mast cells. J Immunol. 2004 Apr 1;172(7):4391-401.
26. Maurer M, Theoharides T, Granstein RD, Bischoff SC, Bienenstock J, Henz B, et al. What is the physiological function of mast cells? Exp Dermatol. 2003 Dec;12(6):886-910.
27. Caughey GH. Mast cell tryptases and chymases in inflammation and host defense. Immunol Rev. 2007 Jun;217:141-54.
28. Souto GR, Queiroz-Junior CM, de Abreu MH, Costa FO, Mesquita RA. Pro-inflammatory, Th1, Th2, Th17 cytokines and dendritic cells: a cross-sectional study in chronic periodontitis. PLoS One. 2014 Mar 26;9(3):e91636. doi: 10.1371/journal.pone.0091636. eCollection 2014.
29. Lamster IB, Grbic JT, Bucklan RS, Mitchell-Lewis D, Reynolds HS, Zambon JJ. Epidemiology and diagnosis of HIV-associated periodontal diseases. Oral Dis. 1997 May;3 Suppl 1:S141-8.
30. Gonçalves LS, Gonçalves BM, Fontes TV. Periodontal disease in HIV-infected adults in the HAART era: Clinical, immunological, and microbiological aspects. Arch Oral Biol. 2013 Oct;58(10):1385-96. doi: 10.1016/j.archoralbio.2013.05.002.
31. Mataftsi M, Skoura L, Sakellari D. HIV infection and periodontal diseases: an overview of the post-HAART era. Oral Dis. 2011 Jan;17(1):13-25. doi: 10.1111/j.1601-0825.2010.01727.x.
32. Ryder MI, Nittayananta W, Coogan M, Greenspan D, Greenspan JS. Periodontal disease in HIV/AIDS. Periodontol 2000. 2012 Oct;60(1):78-97. doi: 10.1111/j.1600-0757.2012.00445.x.
33. Peppes CP, Lemos ASP, Araujo RLF, Portugal MEG, Buffon MCM, Raboni SM. Oral lesions frequency in HIV-positive patients at a tertiary hospital, Southern Brazil. Bra J of Oral Sci. 2013;12(3):216-22. doi: 10.1590/S1677-32252013000300012.
34. Groenewegen H, Bierman WFW, Delli K, Dijkstra PU, Nesse W, Vissink A, et al. Severe periodontitis is more common in HIV- infected patients. J Infect. 2019 Mar;78(3):171-177. doi: 10.1016/j.jinf.2018.11.008.
35. Jiang AP, Jiang JF, Wei JF, Guo MG, Qin Y, Guo QQ, et al. Human Mucosal Mast Cells Capture HIV-1 and Mediate Viral trans-Infection of CD4+ T Cells. J Virol. 2015 Dec 30;90(6):2928-37. doi: 10.1128/JVI.03008-15.
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