Influence of desensitizers agents on the dentin bond strength after one-year water storage




Dentin desensitizing, Dentin sensitivity, Calcium fluoride, Oxalic acid


Aim: Evaluate the effect of adhesives systems combined with desensitizer agents on the microtensile bond strength (μTBS) of a composite resin to dentin. Methods: Cervical dentin of thirty-two human molars were used to simulate hypersensitivity areas. The teeth were divided into four groups (n=8), according to the type of adhesive system and desensitizer agents. No desensitizer was used in the control (Clearfil SE Bond – CS). Two experimental groups were pretreated with either MS Coat Bond (MS) or Biofluorid 12 (BF) immediately prior to bonding with CS. The last group corresponded to Gluma Comfort Bond + Desensitizer (GC) application. After dentin treatments, a composite block was built-up on dentin surface and after 24 hours teeth were serially sectioned to obtain bonded bean specimens. Beams were stored in water for 24 hours or one year. Subsequently, the specimens were submitted to the μTBS test. Data were analyzed by two-way mixed ANOVA and Bonferroni’s test (α = 0.05). Results: At 24 hours, there was no significant difference in μTBS among groups. However, at one year, dentin treated with MS or BF demonstrated significantly lower μTBS of CS to dentin compared to control and GC, which kept their μTBS stable. Conclusion: The effect of MS and BF desensitizer agents on the μTBS of CS to dentin did not reduce the μTBS at 24 hours, but it decreases significantly after one year.


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Author Biographies

Gabriel Nima, State University of Campinas

Department of Restorative Dentistry - Operative Dentistry Division - Piracicaba Dental School, State University of Campinas.

Renata Bacelar Sá, State University of Campinas

Department of Restorative Dentistry - Operative Dentistry Division - Piracicaba Dental School, State University of Campinas.

Marcelo Giannini, State University of Campinas

Department of Restorative Dentistry - Operative Dentistry Division - Piracicaba Dental School, State University of Campinas.


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How to Cite

Nima G, Sá RB, Giannini M. Influence of desensitizers agents on the dentin bond strength after one-year water storage. Braz. J. Oral Sci. [Internet]. 2020Mar.9 [cited 2020Oct.30];190:e201602. Available from:

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