Keywords
Plant extracts
Oils, volatile
Lippia
Antifungal agents
Periodontitis
How to Cite
Abstract
Aim: This study evaluated the chemical composition of Lippia thymoides (Lt) essential oil and its antimicrobial activity against fungal strains of Candida albicans (Ca) and Gram-negative bacteria Prevotella intermedia (Pi) and Fusobacterium nucleatum (Fn). Methods: Lt essential oil was obtained by hydrodistillation apparatus with a modified Clevenger extension. The chemical analysis was analyzed by gas phase chromatography and mass spectrometry on Shimadzu QP 2010 plus. Sample sensitivity evaluation was performed by ABHb-inoculum and culture plates were developed with triphenyltetrazolium chloride, also Fn and Pi samples analysis were in anaerobic environment and Ca sample analysis was performed in aerobic environment. The minimum inhibitory concentration (CIM) was determinated by microdilution in eppendorfs tubes. Results: The chemical analysis showed that Thymol (59,91%) is the main compound found in Lt essential oil, also other antifungal and antimicrobial agents were present γ-terpinene (8.16%), p-cymene (7.29%) and β-caryophyllene (4.49%), Thymol is a central ingredient of many medicinal plants and has a potent fungicidal, bactericidal and antioxidant activity, it has been previously shown to have anti-inflammatory activity against Periodontal Disease (PD) cause can reduces prostanoids, interleukins, leukotrienes levels in periodontium. CIM result Pi was 6.5 μg/mL, Fn was 1.5 μg/mL and Ca was 0.19 μg/mL. Conclusion: The antimicrobial activity of L. thymoides, through the compound Thymol, has been shown promising potential against gram-negative periodontopathogenic bacteria and fungi whose therapeutic arsenal is still very restricted.
References
Hoare A, Marsh P, Diaz P. Ecological therapeutic opportunities for oral diseases. Microbiol Spectr. 2017 Aug;5(4):10.1128/microbiolspec.BAD-0006-2016. doi: 10.1128/microbiolspec.BAD-0006-2016.
Dupuy A, David M, Li L, Heider T, Peterson J, Montano E, et al. Redefining the human oral mycobiome with improved practices in amplicon-based taxonomy: discovery of Malassezia as a prominent commensal. PLoS One. 2014 Mar 10;9(3):e90899. doi: 10.1371/journal.pone.0090899.
Xu X, He J, Xue J, Wang Y, Li K, Zhang K, et al. Oral cavity contains distinct niches with dynamic microbial communities. Environ Microbiol. 2015 Mar;17(3):699-710. doi: 10.1111/1462-2920.12502.
Zhang Y, Wang X, Li H, Ni C, Du Z, Yan F. Human oral microbiota and its modulation for oral health. Biomed Pharmacother. 2018 Mar;99:883-93. doi: 10.1016/j.biopha.2018.01.146.
Kolenbrander P, Palmer R Jr, Periasamy S, Jakubovics N. Oral multispecies biofilm development and the key role of cell-cell distance. Nat Rev Microbiol. 2010 Jul;8(7):471-80. doi: 10.1038/nrmicro2381.
Mark Welch J, Dewhirst F, Borisy G. Biogeography of the oral microbiome: the site-specialist hypothesis. Annu Rev Microbiol. 2019 Sep;73:335-58. doi: 10.1146/annurev-micro-090817-062503.
Mark Welch J, Rossetti B, Rieken C, Dewhirst F, Borisy G. Biogeography of a human 2016 Feb;113(6):E791-800. doi: 10.1073/pnas.1522149113.
Page R, Offenbacher S, Schroeder H, Seymour G, Kornman K. Advances in the pathogenesis of periodontitis: summary of developments, clinical implications and future directions. Periodontol 2000. 1997 Jun;14:216-48. doi: 10.1111/j.1600-0757.1997.tb00199.x.
Tanner A, Kent R Jr, Kanasi E, Lu S, Paster B, Sonis S, et al. Clinical characteristics and microbiota of progressing slight chronic periodontitis in adults. J Clin Periodontol. 2007 Nov;34(11):917-30. doi: 10.1111/j.1600-051X.2007.01126.x.
Hajishengallis E, Parsaei Y, Klein M, Koo H. Advances in the microbial etiology and pathogenesis of early childhood caries. Mol Oral Microbiol. 2017 Feb;32(1):24-34. doi: 10.1111/omi.12152.
Lalla R, Patton L, Dongari-Bagtzoglou A. Oral candidiasis: pathogenesis, clinical presentation, diagnosis and treatment strategies. J Calif Dent Assoc. 2013 Apr;41(4):263-8.
Millsop J, Fazel N. Oral candidiasis. Clin Dermatol. 2016 Jul-Aug;34(4):487-94. doi: 10.1016/j.clindermatol.2016.02.022.
Fernandes T, Bhavsar C, Sawarkar S, D'souza A. Current and novel approaches for control of dental biofilm. Int J Pharm. 2018 Jan;536(1):199-210. doi: 10.1016/j.ijpharm.2017.11.019.
Kuang X, Chen V, Xu X. Novel approaches to the control of oral microbial biofilms. Biomed Res Int. 2018 Dec;2018:6498932. doi: 10.1155/2018/6498932.
Caton J, Armitage G, Berglundh T, Chapple I, Jepsen S, Kornman K, et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Clin Periodontol. 2018 Jun;45 Suppl 20:S1-S8. doi: 10.1111/jcpe.12935.
Yost S, Duran-Pinedo A, Teles R, Krishnan K, Frias-Lopez J. Functional signatures of oral dysbiosis during periodontitis progression revealed by microbial metatranscriptome analysis. Genome Med. 2015 Apr;7(1):27. doi: 10.1186/s13073-015-0153-3. Erratum to: 2015 Oct;7(1):111. doi: 10.1186/s13073-015-0231-6.
Van Dyke T, Sima C. Understanding resolution of inflammation in periodontal diseases: Is chronic inflammatory periodontitis a failure to resolve? Periodontol 2000. 2020 Feb;82(1):205-13. doi: 10.1111/prd.12317.
Socransky S. Microbiology of periodontal disease – present status and future considerations. J Periodontol. 1977 Sep;48(9):497-504. doi: 10.1902/jop.1977.48.9.497.
Munusamy K, Vadivelu J, Tay S. A study on Candida biofilm growth characteristics and its susceptibility to aureobasidin A. Rev Iberoam Micol. 2018;35(2):68-72. doi: 10.1016/j.riam.2017.07.001.
Pretzl B, Sälzer S, Ehmke B, Schlagenhauf U, Dannewitz B, Dommisch H, et al. Administration of systemic antibiotics during non-surgical periodontal therapy-a consensus report. Clin Oral Investig. 2019 Jul;23(7):3073-85. doi: 10.1007/s00784-018-2727-0.
Scheibler E, Garcia M, Medina da Silva R, Figueiredo M, Salum F, Cherubini K. Use of nystatin and chlorhexidine in oral medicine: Properties, indications and pitfalls with focus on geriatric patients. Gerodontology. 2017 Sep;34(3):291-8. doi: 10.1111/ger.12278.
Haas A, Pannuti C, Andrade A, Escobar E, Almeida E, Costa F, et al. Mouthwashes for the control of supragingival biofilm and gingivitis in orthodontic patients: evidence-based recommendations for clinicians. Braz Oral Res. 2014 Jul;28(spe):1-8. doi: 10.1590/1807-3107bor-2014.vol28.0021.
Teughels W, Feres M, Oud V, Martín C, Matesanz P, Herrera D. Adjunctive effect of systemic antimicrobials in periodontitis therapy: a systematic review and meta-analysis. J Clin Periodontol. 2020 Jul;47 Suppl 22:257-81. doi: 10.1111/jcpe.13264.
Amankwaah C, Li J, Lee J, Pascall M. Antimicrobial Activity of Chitosan-Based Films Enriched with Green Tea Extracts on Murine Norovirus, Escherichia coli, and Listeria innocua. Int J Food Sci. 2020 Jul;2020:3941924. doi: 10.1155/2020/3941924.
Warad S, Kolar S, Kalburgi V, Kalburgi N. Lemongrass essential oil gel as a local drug delivery agent for the treatment of periodontitis. Anc Sci Life. 2013 Apr;32(4):205-11. doi: 10.4103/0257-7941.131973.
De Oliveira J, Pinto M, Santana L, Pinto A, di Lenardo D, Vasconcelos D. Biological effects of medicinal plants on induced periodontitis: a systematic review. Int J Dent. 2016;2016:3719879. doi: 10.1155/2016/3719879. Corrigendum to: 2018 Feb 27;2018:5656189. doi: 10.1155/2018/5656189.
Mandras N, Nostro A, Roana J, Scalas D, Banche G, Ghisetti V, et al. Liquid and vapour-phase antifungal activities of essential oils against Candida albicans and non-albicans Candida. BMC Complement Altern Med. 016 Aug;16(1):330. doi: 10.1186/s12906-016-1316-5.
Vlachojannis C, Al-Ahmad A, Hellwig E, Chrubasik S. Listerine® Products: An Update on the Efficacy and Safety. Phytother Res. 2016 Mar;30(3):367-73. doi: 10.1002/ptr.5555.
Moura L, da Silva Neto J, Lopes T, Benjamin S, Brito F, Magalhães F, et al. Ethnobotanic, phytochemical uses and ethnopharmacological profile of genus Cnidoscolus spp. (Euphorbiaceae): a comprehensive overview. Biomed Pharmacother. 2019 Jan;109:1670-9. doi: 10.1016/j.biopha.2018.10.015.
Chua C, Lu B, Lv Y, Gu X, Di Thng A, Zhang H. An optimized band-target entropy minimization for mass spectral reconstruction of severely co-eluting and trace-level components. Anal Bioanal Chem. 2018 Oct;410(25):6549-60. doi: 10.1007/s00216-018-1260-y.
Fan S, Chang J, Zong Y, Hu G, Jia J. GC-MS Analysis of the Composition of the Essential Oil from Dendranthema indicum Var. Aromaticum Using Three Extraction Methods and Two Columns. Molecules. 2018 Mar;23(3):576. doi: 10.3390/molecules23030576.
Zhang Y, Wang Y, Zhu X, Cao P, Wei S, Lu Y. Antibacterial and antibiofilm activities of eugenol from essential oil of Syzygium aromaticum (L.) Merr. & L. M. Perry (clove) leaf against periodontal pathogen Porphyromonas gingivalis. Microb Pathog. 2017 Dec;113:396-402. doi: 10.1016/j.micpath.2017.10.054.
Khoddami M, Sheikh Hosseini M, Hassanshahian M. Antibacterial Activity of Semenovia suffruticosa (Essential Oil) Against Pathogenic Bacteria and Determination of Chemical Composition of Essential Oils by Gas Chromatography-Mass Spectrometry Analysis in Four Regions of Kerman. J Diet Suppl. 2019;16(5):530-40. doi: 10.1080/19390211.2018.1472167.
Chagas AC, Oliveira M, Giglioti R, Santana R, Bizzo HR, Gama P, et al. Efficacy of 11 Brazilian essential oils on lethality of the cattle tick Rhipicephalus (Boophilus) microplus. Ticks Tick Borne Dis. 2016 Apr;7(3):427-32. doi: 10.1016/j.ttbdis.2016.01.001.
Marcial G, de Lampasona M, Vega M, Lizarraga E, Viturro C, Slanis A, et al. Intraspecific variation in essential oil composition of the medicinal plant Lippia integrifolia (Verbenaceae). Evidence for five chemotypes. Phytochemistry. 2016 Feb;122:203-12. doi: 10.1016/j.phytochem.2015.11.004.
Silva F, Menezes P, de Sá P, Oliveira A, Souza E, Almeida J, et al. Chemical composition and pharmacological properties of the essential oils obtained seasonally from Lippia thymoides. Pharm Biol. 2016;54(1):25-34. doi: 10.3109/13880209.2015.1005751.
Okuda T, Kokubu E, Kawana T, Saito A, Okuda K, Ishihara K. Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species. Anaerobe 2012 Feb;18(1):110-6. doi: 10.1016/j.anaerobe.2011.09.003.
Bernard C, Lemoine V, Hoogenkamp M, Girardot M, Krom B, Imbert C. Candida albicans enhances initial biofilm growth of Cutibacterium acnes under aerobic conditions. Biofouling. 2019 Mar;35(3):350-60. doi: 10.1080/08927014.2019.1608966.
Moise P, Sakoulas G, Forrest A, Schentag J. Vancomycin in vitro bactericidal activity and its relationship to efficacy in clearance of methicillin-resistant Staphylococcus aureus bacteremia. Antimicrob Agents Chemother. 2007 Jul;51(7):2582-6. doi: 10.1128/AAC.00939-06.
Quindós G, Gil-Alonso S, Marcos-Arias C, Sevillano E, Mateo E, Jauregizar N, et al. Therapeutic tools for oral candidiasis: Current and new antifungal drugs. Med Oral Patol Oral Cir Bucal. 2019 Mar 1;24(2):e172-80. doi: 10.4317/medoral.22978.
Mohamed A, Lu X, Mounmin F. Diagnosis and Treatment of Esophageal Candidiasis: Current Updates. Can J Gastroenterol Hepatol. 2019 Oct;2019:3585136. doi: 10.1155/2019/3585136.
Lyu X, Zhao C, Yan ZM, Hua H. Efficacy of nystatin for the treatment of oral candidiasis: a systematic review and meta-analysis. Drug Des Devel Ther. 2016 Mar;10:1161-71. doi: 10.2147/DDDT.S100795.
Botelho M, Barros G, Queiroz D, Carvalho C, Gouvea J, Patrus L, et al. Nanotechnology in Phytotherapy: Antiinflammatory Effect of a Nanostructured Thymol Gel from Lippia sidoides in Acute Periodontitis in Rats. Phytother Res. 2016 Jan;30(1):152-9. doi: 10.1002/ptr.5516.
De La Chapa J, Singha P, Lee D, Gonzales C. Thymol inhibits oral squamous cell carcinoma growth via mitochondria-mediated apoptosis. J Oral Pathol Med. 2018 Aug;47(7):674-82. doi: 10.1111/jop.12735.
Juiz P, Lucchese A, Gambari R, Piva R, Penolazzi L, Di Ciano M, et al. Essential oils and isolated compounds from Lippia alba leaves and flowers: antimicrobial activityand osteoclast apoptosis. Int J Mol Med. 2015 Jan;35(1):211-7. doi: 10.3892/ijmm.2014.1995.
Sharifzadeh A, Khosravi A, Shokri H, Shirzadi H. Potential effect of 2-isopropyl-5-methylphenol (thymol) alone and in combination with fluconazole against clinical isolates of Candida albicans, C. glabrata and C. krusei. J Mycol Med. 2018 Jun;28(2):294-9. doi: 10.1016/j.mycmed.2018.04.002.
Arbia L, Chikhi-Chorfi N, Betatache I, Pham-Huy C, Zenia S, Mameri N, et al. Antimicrobial activity of aqueous extracts from four plants on bacterial isolates from periodontitis patients. Environ Sci Pollut Res Int. 2017 May;24(15):13394-404. doi: 10.1007/s11356-017-8942-4.
Ahmad A, Khan A, Manzoor N. Reversal of efflux mediated antifungal resistance underlies synergistic activity of two monoterpenes with fluconazole. Eur J Pharm Sci. 2013 Jan;48(1-2):80-6. doi: 10.1016/j.ejps.2012.09.016.
Reginato C, Bandeira L, Zanette R, Santurio J, Alves S, Danesi C. Antifungal activity of synthetic antiseptics and natural compounds against Candida dubliniensis before and after in vitro fluconazole exposure. Rev Soc Bras Med Trop. 2017;50(1):75-9. doi: 10.1590/0037-8682-0461-2016.
Sköld K, Twetman S, Hallgren A, Yucel-Lindberg T, Modéer T. Effect of a chlorhexidine/thymol-containing varnish on prostaglandin E2 levels in gingival crevicular fluid. Eur J Oral Sci. 1998 Feb;106(1):571-5. doi: 10.1046/j.0909-8836.1998.eos106106.x.
Antunes D, Salvia A, de Araújo R, Di Nicoló R, Koga Ito C, de Araujo M. Effect of green tea extract and mouthwash without alcohol on Candida albicans biofilm on acrylic resin. Gerodontology. 2015 Dec;32(4):291-5. doi: 10.1111/ger.12132.
Emiroğlu Z, Yemiş G, Coşkun B, Candoğan K. Antimicrobial activity of soy edible films incorporated with thyme and oregano essential oils on fresh ground beef patties. Meat Sci. 2010 Oct;86(2):283-8. doi: 10.1016/j.meatsci.2010.04.016.
Li X, Yang X, Deng H, Guo Y, Xue J. Gelatin films incorporated with thymol nanoemulsions: Physical properties and antimicrobial activities. Int J Biol Macromol. 2020 May;150:161-8. doi: 10.1016/j.ijbiomac.2020.02.066.
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