Effect of different concentrations of green tea extract solutions on bonding durability of etch-and-rinse adhesive system to caries affected dentin

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DOI:

https://doi.org/10.20396/bjos.v20i00.8660328

Keywords:

Dentin, Camellia sinensis, Tensile strength, Dental caries

Abstract

Aim: The in vitro study evaluated the effect of different concentrations of green tea extract solution (GT) on the bonding durability of etch-and-rinse adhesive system to caries dentin affected (CAD). Methods: Dentinal surfaces of human third molars were polished and submitted to a microbiological caries induction protocol for 14 days. After removal of the infected dentin layer, the samples were randomly divided into 4 groups (n= 10), according to the concentration of GT solution applied in CAD, after acid etching: 0.05%; 0.2%; 2% and NT (no treatment – control). After application of a etch-and-rinse adhesive system (Adper Single Bond 2, 3M ESPE), composite resin restorations were performed on the dentin. After 24 hours, the resin-dentin blocks were sectioned 1mm2 specimens, which were subjected to the microtensile test immediately or after 6 months of storage in water. Data were submitted to two-way ANOVA for randomized blocks and Tukey test (α= 5%). Results: There was no effect of double interaction (p= 0.934). The application of 0.2% GT promoted a statistically significant increase in dentin bond strength values in comparison to the condition where GT was not used (p=0.012). There was a significant decrease of bond strength after 6 months of storage, regardless of dentin pretreatment (p = 0.007). The G test identified that there was no statistical difference regarding failure mode (p= 0.326). Conclusion: The concentration of 0.2% improved the bond strength of an etch-and-rinse adhesive system to caries affected dentin, however, none of dentin pretreatments could prevent the decrease in bond strength over time.

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Author Biographies

Ricky Rolim de Moura, São Leopoldo Mandic Research Institute

São Leopoldo Mandic Faculty, São Leopoldo Mandic Research Institute.

Fabiana Mantovani Gomes França, São Leopoldo Mandic Research Institute

São Leopoldo Mandic Faculty, São Leopoldo Mandic Research Institute.

Cecilia Pedroso Turssi , São Leopoldo Mandic Research Institute

São Leopoldo Mandic Faculty, São Leopoldo Mandic Research Institute.

Roberta Tarkany Basting, São Leopoldo Mandic Research Institute

São Leopoldo Mandic Faculty, São Leopoldo Mandic Research Institute.

Flavia Lucisano Botelho do Amaral, São Leopoldo Mandic Research Institute

São Leopoldo Mandic Faculty, São Leopoldo Mandic Research Institute.

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Published

2021-05-10

How to Cite

1.
Rolim de Moura R, França FMG, Turssi CP, Basting RT, Amaral FLB do. Effect of different concentrations of green tea extract solutions on bonding durability of etch-and-rinse adhesive system to caries affected dentin. Braz. J. Oral Sci. [Internet]. 2021 May 10 [cited 2023 Feb. 3];20(00):e210328. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8660328

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Original Research

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