Influence of laser deproteinization of acid-etched dentin on marginal microleakage of class V composite restoration




Acid etching, dental, Dentin-bonding agents, Dental leakage, Lasers


Aim: Recent reports indicate that deproteinization of acid-etched dentin surface can extend penetration depth of adhesive agents. The main goal of the present research was to investigate the deproteinization effect of Nd:YAG and diode 940 lasers on acid-etched dentin and microleakage grade in class V composite restorations. Methods: 36 extracted human premolar teeth were selected to make standard buccal and lingual class V cavities. These samples were randomly split into three sub-groups: 1.Control group, in which composite was applied for restoration after etch and bonding process without deproteinization; 2.Nd:YAG laser group, in which the teeth were deproteinized with Nd:YAG laser after etching and painting internal surfaces of cavities with Van Geison stain and then composite restorations applied just as control group; 3.Diode laser group, in which the process was similar to Nd:YAG laser group, but instead, diode 940 laser was irradiated. The teeth were bisected into two equal longitudinal buccal and lingual halves. Marginal microleakage of samples was scored by using a stereomicroscope. Kruskal- Wallis, Mann-Whitney U and Fisher’s statistical tests were employed for analysis of the obtained data. Results: A significant reduction in marginal microleakage was observed for both groups treated with laser (Nd:YAG and diode 940)compared to control (p=0.001 & p=0.047). There was no significant difference in marginal microleakage between Nd:YAG laser and diode 940groups (P = 0.333). Conclusion: Nd:YAG and diode 940 laser deproteinization of acid-etched dentin decreased the marginal microleakage of in-vitro class V resin composite restorations.


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Author Biographies

Shahin Kasraei, Shahid Beheshti University of Medical Sciences

Department of Restorative Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Sogol NejadKarimi, Shahid Beheshti University of Medical Sciences

Department of Restorative Dentistry, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Mona Malek, Tehran University of Medical Science

Dental Clinic of AmirAlam Hospital, Tehran University of Medical Science, Tehran, Iran.

Mahsa Mohammadi, Hamedan University of Medical Sciences

Dental School, Hamedan University of Medical Sciences, Hamedan, Iran.


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How to Cite

Kasraei S, NejadKarimi S, Malek M, Mohammadi M. Influence of laser deproteinization of acid-etched dentin on marginal microleakage of class V composite restoration. Braz. J. Oral Sci. [Internet]. 2021Feb.17 [cited 2022Jan.20];20(00):e210525. Available from:



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