Randomized clinical trial of complete denture cleaning methods in residents of care institutions





Dentures, Hygiene, Biofilms, Health services for the aged, Elderly


Aim: To evaluate the efficacy of ultrasonic bath and traditional brushing in the hygiene of complete dentures of dependent residents in long-term care institutions (LTCIs). Methods: A randomized cross-over clinical study was conducted in 17 maxillary complete denture wearers living in LTCIs. Cleaning protocols were brushing or ultrasonic bath, both applied with neutral liquid soap. Biofilm biomass were estimated by MTT reduction assay and specific microbial load (CFU) of Streptococcus spp., Staphylococcus spp. and Candida spp. were quantified by selective and differential culture media. Results: Ultrasound method showed higher percentage reduction of biofilm biomass and specific microbial loads of Streptococcus spp. compared to brushing (p<0.01). Reduction of microbial loads of the other microorganisms were not different between cleaning methods (p>0.05). Conclusions: The ultrasonic bath proved to be a feasible alternative method for the mechanical cleaning of complete dentures in LTCIs.


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Author Biographies

Ana Paula Sponchiado, Pontifical Catholic University of Paraná

Pontifical Catholic University of Paraná. Curitiba, PR, Brazil.

Maria Cecília Carlos Lopes Giacomel, Positivo University

Positivo University. Curitiba, PR, Brazil.

Evelise Machado de Souza, Pontifical Catholic University of Paraná

Pontifical Catholic University of Paraná. Curitiba, PR, Brazil.

Rosimeire Takaki Rosa, Pontifical Catholic University of Paraná

Pontifical Catholic University of Paraná. Curitiba, PR, Brazil.

Edvaldo Antonio Ribeiro Rosa, Pontifical Catholic University of Paraná

Pontifical Catholic University of Paraná. Curitiba, PR, Brazil.

Rodrigo Nunes Rached, Pontifical Catholic University of Paraná

Pontifical Catholic University of Paraná. Curitiba, PR, Brazil.


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How to Cite

Sponchiado AP, Giacomel MCCL, Souza EM de, Rosa RT, Rosa EAR, Rached RN. Randomized clinical trial of complete denture cleaning methods in residents of care institutions. Braz. J. Oral Sci. [Internet]. 2021 Jun. 17 [cited 2022 Dec. 5];20(00):e211060. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8661060



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