Keywords
Dentin
Endodontics
Ethanol
Wettability
Resin cements
How to Cite
Abstract
Aim: The aim of this study was to evaluate the effect of ethanol-conditioned dentin on endodontic sealer penetration into dentinal tubules by confocal laser scanning microscopy (CLSM). Methods: Forty human maxillary anterior teeth were instrumented and divided into four groups (n = 10) according to the drying methods: 1) wet: vacuum only, 2) paper points: vacuum + absorbent paper points, (3) 70% ethanol: 70% ethanol (1 min) + vacuum + absorbent paper points, and (4) 100% ethanol: 100% ethanol (1 min) + vacuum + absorbent paper points. All root canals were filled with resin-based endodontic sealer. Four sections from each third (cervical, middle, and apical) were examined by CLSM. Root canal wall perimeter infiltrated by sealer, maximum depth of sealer penetration, percentage of penetrated area, and fluorescence intensity of rhodamine B were measured. Statistical analysis was performed by analysis of variance and Tukey’s tests (α = 0.05). Results: No statistical difference was found when percentage of root canal wall coverage infiltrated by sealer were compared. The groups in which ethanol solutions were used presented greater depth of sealer penetration, higher percentage of penetrated area, and higher fluorescence intensity of rhodamine B (p< 0.05) when compared with the wet and paper point groups. Overall, 100% ethanol produced better results than 70% ethanol, except for rhodamine B intensity (cervical third). In addition, the absorbent paper points drying method behaved better than did vacuum only group, except for rhodamine B intensity (apical third). Conclusion: Ethanol-conditioned dentin improved the penetration of resin-based sealer into dentinal tubules, especially at the concentration of 100%.
References
Ricucci D, Siqueira Jr JF. Biofilms and apical periodontitis: study of prevalence and association with clinical and histopathologic findings. J Endod. 2010 Aug;36(8):1277-88. doi: 10.1016/j.joen.2010.04.007.
Rosales JI, Marshall GW, Marshall SJ, Watanabe LG, Toledano M, Cabrerizo MA, et al. Acid-etching and hydration influence on dentin roughness and wettability. J Dent Res. 1999 Sep;78(9):1554-9. doi: 10.1177/0022034599078009100.
Mamootil K, Messer HH. Penetration of dentinal tubules by endodontic sealer cements in extracted teeth and in vivo. Int Endod J. 2007 Nov;40(11):873-81. doi: 10.1111/j.1365-2591.2007.01307.x.
Kara Tuncer A, Tuncer S. Effect of different final irrigation solutions on dentinal tubule penetration depth and percentage of root canal sealer. J Endod. 2012 Jun;38(6):860-3. doi: 10.1016/j.joen.2012.03.008.
Wang Y, Liu S, Dong Y. In vitro study of dentinal tubule penetration and filling quality of bioceramic sealer. Plos One. 2018 Feb;13(2):e0192248. doi: 10.1371/journal.pone.0192248.
Hashem AA, Ghoneim AG, Lutfy RA, Fouda MY. The effect of different irrigating solutions on bond strength of two root canal-filling systems. J Endod. 2009 Apr;35(4):537-40. doi: 10.1016/j.joen.2009.01.003.
Moradi S, Ghoddusi J, Forghani M. Evaluation of dentinal tubule penetration after the use of dentin bonding agent as a root canal sealer. J Endod. 2009 Nov;35(11):1563-6. doi: 10.1016/j.joen.2009.08.001.
Tay FR, Pashley DH, Kapur RR, Carrilho MR, Hur YB, Garrett LV, et al. Bonding BisGMA to dentin: a proof of concept for hydrophobic dentin bonding. J Dent Res. 2007 Nov;86(11):1034-9. doi: 10.1177/154405910708601103.
D’Alpino PH, Pereira JC, Svizero NR, Rueggeberg FA, Pashley DH. Use of fluorescent compounds in assessing bonded resin-based restorations: a literature review. J Dent. 2006 Oct;34(9):623-34. doi: 10.1016/j.jdent.2005.12.004.
Stevens RW, Strother JM, McClanahan SB. Leakage and sealer penetration in smear-free dentin after a final rinse with 95% ethanol. J Endod. 2006 Aug;32(8):785-8. doi: 10.1016/j.joen.2006.02.027.
Ricucci D, Siqueira JF Jr, Bate AL, Pitt Ford TR. Histologic investigation of root canal-treated teeth with apical periodontitis: a retrospective study from twenty-four patients. J Endod. 2009 Apr;35(4):493-502. doi: 10.1016/j.joen.2008.12.014.
Vassiliadis LP, Sklavounos SA, Stavrianos CK. Depth of penetration and appearance of Grossman sealer in the dentinal tubules: an in vivo study. J Endod. 1994 Aug;20(8):373-6. doi: 10.1016/S0099-2399(06)80293-1.
Carvalho CA, Monticelli F, Cantoro A, Breschi L, Ferrari M. Push-out bond strength of fiber posts luted with unfilled resin cement. J Adhes Dent. 2009 Feb;11(1):65-70.
Carvalho CA, Breschi L, Navarro MF, Atta MT, Ferrari M. Push-out bond strength and SEM evaluation of a new bonding approach into the root canal. J Appl Oral Sci. 2012;20(6):613-9. doi: 10.1590/s1678-77572012000600005.
Pantoja CAMS, Silva DHD, Soares AJ, Ferraz CCR, Gomes BPFA, Zaia AA, et al. Influence of ethanol on dentin roughness, surface free energy, and interaction between AH Plus and root dentin. Braz Oral Res. 2018;32:e33. doi: 10.1590/1807-3107bor-2018.vol32.0033.
Ordinola-Zapata R, Bramante CM, Graeff MS, del Carpio Perochena A, Vivan RR, Camargo EJ, et al. Depth and percentage of penetration of endodontic sealers into dentinal tubules after root canal obturation using a lateral compaction technique: a confocal laser scanning microscopy study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009 Sep;108(3):450-7. doi: 10.1016/j.tripleo.2009.04.024.
Gibby SG, Wong Y, Kulild JC, Williams KB, Yao X, Walker MP. Novel methodology to evaluate the effect of residual moisture on epoxy resin sealer/dentine interface: a pilot study. Int Endod J. 2011 Mar;44(3):236-44. doi: 10.1111/j.1365-2591.2010.01806.x.
Wang Y, Spencer P. Continuing etching of an all-in-one adhesive in wet dentin tubules. J Dent Res. 2005 Apr;84(4):350-4. doi: 10.1177/154405910508400411.
Calt S, Serper A. Dentinal tubule penetration of root canal sealers after root canal dressing with calcium hydroxide. J Endod. 1999 Jun;25(6):431-3. doi: 10.1016/S0099-2399(99)80273-8.
Moon YM, Shon WJ, Baek SH, Bae KS, Kum KY, Lee W. Effect of final irrigation regimen on sealer penetration in curved root canals. J Endod. 2010 Apr;36(4):732-6. doi: 10.1016/j.joen.2009.12.006.
Pei D, Huang X, Huang C, Wang Y, Ouyang X, Zhang J. Ethanol-wet bonding may improve root dentine bonding performance of hydrophobic adhesive. J Dental Res. 2012 May;40(5):433-41. doi: 10.1016/j.jdent.2012.02.005.
Chandra SS, Shankar P, Indira R. Depth of penetration of four resin sealers into radicular dentinal tubules: confocal microscopic study. J Endod. 2012 Oct;38(10):1412-6. doi: 10.1016/j.joen.2012.05.017.
Ekambaram M, Yiu C, Matinlinna J. Bonding of adhesive resin to intraradicular dentine: A review of the literature. Int J Adhes Adhes. 2015;60:92-103. doi: 10.1016/j.ijadhadh.2015.04.003.
Patel DV, Sherriff M, Ford TR, Watson TF, Mannocci F. The penetration of RealSeal primer and Tubliseal into root canal dentinal tubules: a confocal microscopic study. Int Endod J. 2007 Jan;40(1):67-71. doi: 10.1111/j.1365-2591.2006.01184.x.
Zmener O, Pameijer CH, Serrano SA, Vidueira M, Macchi RL. Significance of moist root canal dentin with the use of methacrylate-based endodontic sealers: an in vitro coronal dye leakage study. J Endod. 2008 Jan;34(1):76-9. doi: 10.1016/j.joen.2007.10.012.
Generali L, Cavani F, Serena V, Pettenati C, Righi E, Bertoldi C. Effect of different irrigation systems on sealer penetration into dentinal tubules. J Endod. 2017 Apr;43(4):652-656. doi: 10.1016/j.joen.2016.12.004.
Wilcox LR, Wiemann AH. Effect of a final ethanol rinse on sealer coverage of obturated root canals. J Endod. 1995 May;21(5):256-8. doi: 10.1016/s0099-2399(06)80992-1.
Almeida JF, Gomes BP, Ferraz CC, Souza-Filho FJ, Zaia AA. Filling of artificial lateral canals and microleakage and flow of five endodontic sealers. Int Endod J. 2007 Sep;40(9):692-9. doi: 10.1111/j.1365-2591.2007.01268.x.
Picoh T, Stotz S, Buff E, Duschner H, Staehle HJ. Influence of different etching times on hybrid layer formation and tensile bond strength. American J Dent. 1998 Oct;11(5):202-6.
Zhang H, Shen Y, Ruse ND, Haapasalo M. Antibacterial activity of endodontic sealers by modified direct contact test against Enterococcus faecalis. J Endod. 2009 Jul;35(7):1051-5. doi: 10.1016/j.joen.2009.04.022.
Heling I, Chandler NP. The antimicrobial effect within dentinal tubules of four root canal sealers. J Endod. 1996 May;22(5):257-9. doi: 10.1016/s0099-2399(06)80144-5.
Ordinola-Zapata R, Bramante CM, Cavenago B, Graeff MS, Gomes de Moraes I, Marciano M, et al. Antimicrobial effect of endodontic solutions used as final irrigants on a dentine biofilm model. Int Endod J. 2012 Feb;45(2):162-8. doi: 10.1111/j.1365-2591.2011.01959.x.
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