Influence of rs6667202 SNP on Interleukin-10 levels in the gingival fluid of patients with periodontitis grade C

Authors

DOI:

https://doi.org/10.20396/bjos.v20i00.8661654

Keywords:

Interleukin-10, Aggressive periodontitis, Polymorphism, single nucleotide

Abstract

Grade C periodontitis in youngers is characterized by a severe form of periodontitis, and IL10 rs6667202 single nucleotide polymorphism (SNP) has been described as an important feature in this disease etiology. Aim: This study aimed to evaluate, in vivo, the functionality of IL10 rs6667202 SNP on IL-10 gingival fluid levels. Methods: Thirty patients with Perio4C were selected, 15 with the IL10 AA genotype (rs6667202) and 15 with AC/CC genotypes. The gingival fluid was collected from two sites with probing depth ≥ 7 mm and bleeding on probing, and two healthy sites. The IL-10 concentration was determined by Luminex/MAGpix platform. Results: In deep pockets, the IL10 AA genotype presented a lower concentration of IL-10 when compared with AC or CC genotypes (p<0.05). In shallow pockets, no difference between groups was seen (p>0.05). Conclusion: IL10 rs6667202 SNP decreases the production of IL-10 in crevicular fluid, potentially affecting this disease progression.  

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

Author Biographies

Camila Schmidt Stolf, University of Campinas

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

Tiago Taiete, University of Campinas. University of Araras.

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

Department of Dentistry, University of Araras, Araras, SP, Brazil

Márcio Zaffalon Casati, University of Campinas. Paulista University.

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

Department of Periodontics, Paulista University, São Paulo, SP, Brazil.

Enílson Antônio Sallum, University of Campinas

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

Francisco Humberto Nociti Júnior, University of Campinas

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

Karina Gonzales Silvério Ruiz, University of Campinas

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

RenatoCorrêa Viana Casarin, University of Campinas

Department of Prosthodontics and Periodontics, Periodontics Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil.

References

Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999 Dec;4(1):1-6. doi: 10.1902/annals.1999.4.1.1.

Albandar JM. Aggressive and acute periodontal diseases. Periodontol 2000. 2014 Jun;65(1):7-12. doi: 10.1111/prd.12013.

Caton JG, Armitage G, Berglundh T, Chapple ILC, Jepsen S, Kornman KS, et al. A new classification scheme for periodontal and peri-implant diseases and conditions - Introduction and key changes from the 1999 classification. J Periodontol. 2018 Jun;89 Suppl 1:S1-S8. doi:10.1002/JPER.18-0157.

Tonetti MS, Mombelli A. Early-onset periodontitis. Ann Periodontol. 1999 Dec;4(1):39-53. doi: 10.1902/annals.1999.4.1.39.

Christersson LA, Slots J, Rosling BG, Genco RJ. Microbiological and clinical effects of surgical treatment of localized juvenile periodontitis. J Clin Periodontol. 1985 Jul;12(6):465-76. doi: 10.1111/j.1600-051x.1985.tb01382.x.

Deas DE, Mealey BL. Response of chronic and aggressive periodontitis to treatment. Periodontol 2000. 2010 Jun;53:154-66. doi:10.1111/j.1600-0757.2009.00334.x. Review.

Scharf S, Wohlfeil M, Siegelin Y, Schacher B, Dannewitz B, Eickholz P. Clinical results after nonsurgical therapy in aggressive and chronic periodontitis. Clin Oral Investig. 2014;18(2):453-60. doi:10.1007/s00784-013-1013-4.

Teughels W, Dhondt R, Dekeyser C, Quirynen M. Treatment of aggressive periodontitis. Periodontol 2000. 2014 Jun;65(1):107-33. doi: 10.1111/prd.12020.

Guerrero A, Griffiths GS, Nibali L, Suvan J, Moles DR, Laurell L, et al. Adjunctive benefits of systemic amoxicillin and metronidazole in non-surgical treatment of generalized aggressive periodontitis: a randomized placebo-controlled clinical trial. J Clin Periodontol. 2005 Oct;32(10):1096-107. doi: 10.1111/j.1600-051X.2005.00814.x.

Bastos MF, Lima JA, Vieira PM, Mestnik MJ, Faveri M, Duarte PM. TNF-alpha and IL-4 levels in generalized aggressive periodontitis subjects. Oral Dis. 2009 Jan;15(1):82-7. doi: 10.1111/j.1601-0825.2008.01491.x.

Duarte PM, da Rocha M, Sampaio E, Mestnik MJ, Feres M, Figueiredo LC, et al. Serum levels of cytokines in subjects with generalized chronic and aggressive periodontitis before and after non-surgical periodontal therapy: a pilot study. J Periodontol. 2010 Jul;81(7):1056-63. doi: 10.1902/jop.2010.090732.

Shaddox L, Wiedey J, Bimstein E, Magnuson I, Clare-Salzler M, Aukhil I, et al. Hyper-responsive phenotype in localized aggressive periodontitis. J Dent Res. 2010 Feb;89(2):143-8. doi: 10.1177/0022034509353397.

Loos BG, Papantonopoulos G, Jepsen S, Laine ML. What is the contribution of genetics to periodontal risk? Dent Clin North Am. 2015 Oct;59(4):761-80. doi: 10.1016/j.cden.2015.06.005.

Loos BG, John RP, Laine ML. Identification of genetic risk factors for periodontitis and possible mechanisms of action. J Clin Periodontol. 2005;32 Suppl 6:159-79. doi: 10.1111/j.1600-051X.2005.00806.x.

Nibali L, Pelekos G, D'Aiuto F, Chaudhary N, Habeeb R, Ready D, Parkar M, et al. Influence of il-6 haplotypes on clinical and inflammatory response in aggressive periodontitis. Clin Oral Investig. 2013 May;17(4):1235-42. doi: 10.1007/s00784-012-0804-3.

Taiete T, Casati MZ, Stolf CS, Corrêa MG, Santamaria MP, Andere NMRB, et al. Validation of reported GLT6D1 (rs1537415), IL10 (rs6667202), and ANRIL (rs1333048) single nucleotide polymorphisms for aggressive periodontitis in a Brazilian population. J Periodontol. 2019 Jan;90(1):44-51. doi: 10.1002/JPER.18-0071.

Schaefer AS, Bochenek G, Manke T, Nothnagel M, Graetz C, Thien A, et al. Validation of reported genetic risk factors for periodontitis in a large-scale replication study. J Clin Periodontol. 2013 Jun;40(6):563-72. doi: 10.1111/jcpe.12092.

Garlet GP. Destructive and protective roles of cytokines in periodontitis: a re-appraisal from host defense and tissue destruction viewpoints. J Dent Res. 2010 Dec;89(12):1349-63. doi: 10.1177/0022034510376402.

Garlet GP, Cardoso CR, Campanelli AP, Martins W Jr, Silva JS. Expression of suppressors of cytokine signaling in diseased periodontal tissues: a stop signal for disease progression? J Periodontal Res. 2006 Dec;41(6):580-4. doi: 10.1111/j.1600-0765.2006.00908.x.

Casarin RC, Ribeiro Edel P, Mariano FS, Nociti FH Jr, Casati MZ, Gonçalves RB. Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis. J Periodontal Res. 2010 Oct;45(5):635-42. doi: 10.1111/j.1600-0765.2010.01278.x.

Teles RP, Gursky LC, Faveri M, Rosa EA, Teles FR, Feres M, et al. Relationships between subgingival microbiota and GCF biomarkers in generalized aggressive periodontitis. J Clin Periodontol. 2010 Apr;37(4):313-23. doi: 10.1111/j.1600-051X.2010.01534.x.

Mattuella LG, Campagnaro MB, Vargas AE, Xavier LL, Oppermann RV, Chies JA, et al. Plasma cytokines levels in aggressive and chronic periodontitis. Acta Odontol Scand. 2013 May-Jul;71(3-4):683-8. doi: 10.3109/00016357.2012.715191.

Trevilatto PC, Line SR. Use of buccal epithelial cells for PCR amplification of large DNA fragments. J Forensic Odontostomatol. 2000 Jun;18(1):6-9.

Albandar JM, Tinoco E. Global epidemiology of periodontal diseases in children and young persons. Periodontol 2000. 2002;29:153-76. doi: 10.1034/j.1600-0757.2002.290108.x.

Susin C, Albandar JM. Aggressive periodontitis in an urban population in southern Brazil. J Periodontol. 2005 Mar;76(3):468-75. doi: 10.1902/jop.2005.76.3.468.

Susin C, Haas AN, Albandar JM. Epidemiology and demographics of aggressive periodontitis. Periodontol 2000. 2014 Jun;65(1):27-45. doi: 10.1111/prd.12019.

Laine ML, Crielaard W, Loos BG. Genetic susceptibility to periodontitis. Periodontol 2000. 2012 Feb;58(1):37-68. doi: 10.1111/j.1600-0757.2011.00415.x.

Schaefer AS, Richter GM, Nothnagel M, Manke T, Dommisch H, Jacobs G, et al. A genome-wide association study identifies GLT6D1 as a susceptibility locus for periodontitis. Hum Mol Genet. 2010 Feb 1;19(3):553-62. doi: 10.1093/hmg/ddp508.

Vieira AR, Albandar JM. Role of genetic factors in the pathogenesis of aggressive periodontitis. Periodontol 2000. 2014 Jun;65(1):92-106. doi: 10.1111/prd.12021.

Schaefer AS, Richter GM, Groessner-Schreiber B, Noack B, Nothnagel M, El Mokhtari NE, et al. Identification of a shared genetic susceptibility locus for coronary heart disease and periodontitis. PLoS Genet. 2009;5(2):e1000378. doi:10.1371/journal.pgen.1000378.

Cortelli JR, Cortelli SC, Jordan S, Haraszthy VI, Zambon JJ. Prevalence of periodontal pathogens in Brazilians with aggressive or chronic periodontitis. J Clin Periodontol. 2005 Aug;32(8):860-6. doi: 10.1111/j.1600-051X.2005.00777.x.

Könönen E, Müller HP. Microbiology of aggressive periodontitis. Periodontol2000. 2014 Jun;65(1):46-78. doi: 10.1111/prd.12016.

Houri-Haddad Y, Soskolne WA, Halabi A, Shapira L. IL-10 gene transfer attenuates P. gingivalis-induced inflammation. J Dent Res. 2007 Jun;86(6):560-4. doi: 10.1177/154405910708600614.

Marinou I, Healy J, Mewar D, Moore DJ, Dickson MC, Binks MH, et al. Association of interleukin-6 and interleukin-10 genotypes with radiographic damage in rheumatoid arthritis is dependent on autoantibody status. Arthritis Rheum. 2007 Aug;56(8):2549-56. doi: 10.1002/art.22814.

Reichert S, Machulla HK, Klapproth J, Zimmermann U, Reichert Y, Gläser CH, et al. The interleukin-10 promoter haplotype ATA is a putative risk factor for aggressive periodontitis. J Periodontal Res. 2008 Feb;43(1):40-7. doi: 10.1111/j.1600-0765.2007.00992.x.

Downloads

Published

2021-02-08

How to Cite

1.
Stolf CS, Taiete T, Zaffalon Casati M, Sallum EA, Nociti Júnior FH, Ruiz KGS, Casarin RV. Influence of rs6667202 SNP on Interleukin-10 levels in the gingival fluid of patients with periodontitis grade C. Braz. J. Oral Sci. [Internet]. 2021 Feb. 8 [cited 2022 Dec. 9];20(00):e211654. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8661654

Issue

Section

Original Research

Most read articles by the same author(s)