Cleaning of long oval canals with WaveOne Gold system associated with different irrigant agitation protocols




Endodontics, Root canal irrigants, Root canal preparation, Microscopy, electron, scanning


Aim: The aim of the study was to evaluate the cleaning of mandibular incisors with WaveOne Gold® (WO) under different preparation techniques. Methods: A total of 210 human mandibular incisors were selected and divided into seven groups (n = 30), prepared by WO single-files (Small 20/.07 – WOS; Primary 25/.07 – WOP; Medium 35/.06 – WOM; or Large 45/.05 - WOL) and sequential-file techniques (WOS to WOP; WOS to WOM; and WOS to WOL). Further subdivision was made according to irrigation protocol: control group (manual irrigation - CON), E1 Irrisonic® - EIR, and EDDY® - EDD. Debris removal and the smear layer were evaluated by scanning electron microscopy. Data were analyzed by using Spearman’s correlation test. The significance level was set at 5%. Results: For debris and smear layer removal, WOS and WOP, EIR differed from CON and EDD (p <0.05). Conclusion: Regardless of the instrumentation used, the agitation of the irrigant solution provided better cleanability. These findings reinforce the need for agitation techniques as adjuvants in cleaning root canal systems in mandibular incisors.


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Author Biographies

Prescila Mota de Oliveira Kublitski, Positivo University

School of Health Sciences, Positivo University, Curitiba, Brazil.

Flávia Sens Fagundes Tomazinho, Positivo University

School of Health Sciences, Positivo University, Curitiba, Brazil.

Bruno Marques-da-Silva, Positivo University

School of Health Sciences, Positivo University, Curitiba, Brazil.

Vinícius Rodrigues dos Santos, University of Campinas

Doctoral Student (Prosthesis and Periodontics) Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.

Wander José da Silva, University of Campinas

Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.

Flares Baratto-Filho, University of Joinville

Teacher at Department of Operative Dentistry, School of Dentistry, University of Joinville, Joinville, Santa Catarina, Brazil.

Marilisa Carneiro Leão Gabardo, Positivo University

Teacher from the School of Health Sciences, Positivo University, Curitiba, Brazil.


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How to Cite

Kublitski PM de O, Tomazinho FSF, Marques-da-Silva B, Santos VR dos, Silva WJ da, Baratto-Filho F, Gabardo MCL. Cleaning of long oval canals with WaveOne Gold system associated with different irrigant agitation protocols . Braz. J. Oral Sci. [Internet]. 2021 Aug. 24 [cited 2022 Aug. 18];21(00):e224013. Available from:



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