Keywords
Tea
Chitosan
Tooth erosion
Nanoparticles
How to Cite
Abstract
Aim: To evaluate the influence of the biomodification of erosive lesions with a chitosan nanoformulation containing green tea (NanoCsQ) on the clinical performance of a composite resin. Methods: The study was performed in a split-mouth, randomized and double-blinded model with 20 patients with 40 erosive lesions. The patient’s teeth were randomized into two groups (n=20) according to the surface treatment: 1) Without biomodification (control), and 2) Biomodification with NanoCsQ solution (experimental). The lesions were restored with adhesive (Tetric N-bond, Ivoclar) and composite resin (IPS Empress Direct, Ivoclar). The restorations were polished and 7 days (baseline), 6 months, and 12 months later were evaluated according to the United States Public Health Service (USPHS) modified criteria, using clinical exam and photographics. Data were analyzed by Friedman’s and Wilcoxon signed-rank tests. Results: No significant differences were found between the control and experimental groups (p=0.423), and also among the follow-up periods (baseline, six months, and 12 months) (p=0.50). Regarding the retention criteria, 90% of the restoration had an alpha score in the control group. Only 10% of the restorations without biomodification (control) had a score charlie at the 12-month follow-up. None of the patients reported post-operatory sensitivity. Conclusion: The NanoCsQ solution did not negatively affect the performance of the composite resin restorations after 12 months.
References
Warreth A, Abuhijleh E, Almaghribi MA, Mahwal G, Ashawish A. Tooth surface loss: a review of the literature. Saudi Dent J. 2020 Feb;32(2):53-60. doi: 10.1016/j.sdentj.2019.09.004.
Wilder AD Jr, Swift EJ Jr, Heymann HO, Ritter AV, Sturdevant JR, Bayne SC. A 12-year clinical evaluation of a three-step dentin adhesive in noncarious cervical lesions. J Am Dent Assoc. 2009 May;140(5):526-35. doi: 10.14219/jada.archive.2009.0220.
Vailati F, Gruetter L, Belser UC. Adhesively restored anterior maxillary dentitions affected by severe erosion: up to 6-year results of a prospective clinical study. Eur J Esthet Dent. 2013;8(4):506-30.
Zimmerli B, De Munck J, Lussi A, Lambrechts P, Van Meerbeek B. Longterm bonding to eroded dentin requires superficial bur preparation. Clin Oral Invest. 2012 Oct;16(5):1451-61. doi: 10.1007/s00784-011-0650-8.
de Souza Costa CA, Hebling J, Scheffel DL, Soares DG, Basso FG, Ribeiro AP. Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques. Dent Mater. 2014 Jul;30(7):769-84. doi: 10.1016/j.dental.2014.04.010.
Fakhri E, Eslami H, Maroufi P, Pakdel F, Taghizadeh S, Ganbarov K, et al. Chitosan biomaterials application in dentistry. Int J Biol Macromol. 2020 Nov;162:956-74. doi: 10.1016/j.ijbiomac.2020.06.211.
Muzzarelli C, Muzzarelli RA. Natural and artificial chitosan-inorganic composites. J Inorg Biochem. 2002 Nov;92(2):89-94. doi: 10.1016/s0162-0134(02)00486-5.
Pimenta JA, Zaparolli D, Pécora JD, Cruz-Filho AM. Chitosan: effect of a new chelating agent on the microhardness of root dentin. Braz Dent J. 2012;23(3):212-7. doi: 10.1590/s0103-64402012000300005.
Husain S, Al-Samadani KH, Najeeb S, Zafar MS, Khurshid Z, Zohaib S, et al. Chitosan biomaterials for current and potential dental applications. Materials (Basel). 2017 May;10(6):602. doi: 10.3390/ma10060602.
Elsaka SE, Elnaghy AM. Antibacterial activity of calcium hydroxide combined with chitosan solutions and the outcomes on the bond strength of Real Sealer sealer to radicular dentin. J Biomed Res. 2012 May;26(3):193-9. doi: 10.7555/JBR.26.20110136.
Rabea EI, Badawy ME, Stevens CV, Smagghe G, Steurbaut W. Chitosan as antimicrobial agent: applications and mode of action. Biomacromolecules. 2003 Nov-Dec;4(6):1457-65. doi: 10.1021/bm034130m.
Silva PV, Guedes DF, Pecora JD, Cruz-Filho AM. Time-dependent effects of chitosan on dentin structures. Braz Dent J. 2012;23(4):357-61. doi: 10.1590/s0103-64402012000400008.
Profeta AC, Mannocci F, Foxton RM, Thompson I, Watson TF, Sauro S. Bioactive effects of a calcium/sodium phosphosilicate on the resin-dentine interface: a micro tensile bond strength, scanning electron microscopy, and confocal microscopy study. Eur J Oral Sci. 2012 Aug;120(4):353-62. doi: 10.1111/j.1600-0722.2012.00974.x.
Fawzy AS, Nitisusanta LI, Iqbal K, Daood U, Beng, LT, Neo J. Chitosan/Riboflavin-modified demineralized dentin as a potential substrate for bonding. J Mech Behav Biomed Mater. 2013 Jan;17:278-89. doi: 10.1016/j.jmbbm.2012.09.008.
Bedran-Russo AK, Pereira PN. The application of crosslinkers to dentin collagen enhances the ultimate tensilstrenght. J Biomed Mater Res B Appl Biomater. 2007 Jan;80(1):268-72. doi: 10.1002/jbm.b.30593.
Shrestha A, Friedman S, Kishen A. Photodynamically crosslinked and chitosan-incorporated dentin collagen. J Dent Res. 2011 Nov;90(11):1346-51. doi: 10.1177/0022034511421928.
Dash M, Chiellini F, Ottenbrite RM, Chiellini E. Chitosan-A versatile semi-synthetic polymer in biomedical applications. Prog Polym Sci. 2011 Aug;36(8):981-1014. doi: 10.1016/j.progpolymsci.2011.02.001.
Tjäderhane L, Nascimento FD, Breschi L, Mazzoni A, Tersariol IL, Geraldeli S, et al. Optimizing dentin bond durability: strategies to prevent hydrolytic degradation of the hybrid layer. Dent Mater. 2013 Oct;29(10):999-1011. doi: 10.1016/j.dental.2013.07.016.
Amaral RC, Solari, HP. Collagen crosslinking for the treatment of keratoconus. Rev Bras Oftalmol. 2009;68(6):359-64. doi: 10.1590/S0034-72802009000600008.
Nishimura M, Ishiyama K, Watanabe A. Determination of theaflavins including methylated theaflavins in black tea leaves by solid-phase extraction and HPLC analysis. J Agric Food Chem. 2007 Sep;55(18):7252-7. doi: 10.1021/jf070312m.
Aguiar TR, Vidal CM, Phansalkar RS. Dentin biomodification potential depends on the polyphenol source. J Dent Res. 2014 Apr;93(4):417-22. doi: 10.1177/0022034514523783.
Chu C, Deng J, Man Y, Qu Y. Green Tea Extracts Epigallocatechin-3-gallate for Different Treatments. Biomed Res Int. 2017;2017:5615647. doi: 10.1155/2017/5615647.
Santiago SL, Osorio R, Neri JR, Carvalho RM, Toledano M. Effect of the flavonoid epigallocatechin-3-gallate on resin-dentin bond strength. J Adhes Dent. 2013 Dec;15(6):535-40. doi: 10.3290/j.jad.a29532.
Shpigelman A, Cohen Y, Livney YD. Thermally-induced b-lactoglobulin-EGCG nanovehicles: loading, stability, sensory and digestive-release study. Food Hidrocoll. 2012 Oct;29(1):57-67. doi: 10.1016/j.foodhyd.2012.01.016.
Sasaki H, Matsumoto M, Tanaka T, Maeda M, Nakai M, Hamada S, et al. Antibacterial activity of polyphenol components in oolong tea extract against Streptococcus mutants. Caries Res. 2004 Jan-Feb;38(1):2-8. doi: 10.1159/000073913.
Xu Z, Neoh KG, Lin CC, Kishen A. Biomimetic deposition of calcium phosphate minerals on the surface of partially demineralized dentine modified with phosphorylated chitosan. J Biomed Mater ResB Appl Biomater. 2011 Jul;98(1):150-9. doi: 10.1002/jbm.b.31844.
Souza JC, Tedesco AC, Takahashi LAU, Curylofo-Zotti FA, Souza-Gabriel AE, Corona SAM. Influence of nanoparticulated chitosan on the biomodification of eroded dentin: clinical and photographic longitudinal analysis of restorations. J Mater Sci Mater Med. 2021 Jan;32(1):11. doi: 10.1007/s10856-020-06487-2.
Curylofo-Zotti FA, Tedesco AC, Lizarelli GTC, Takahashi LAU, Corona SAM. Effect of green tea-loaded chitosan nanoparticles on leathery dentin microhardness. Odontology. 2021 Oct;109(4):860-7. doi: 10.1007/s10266-021-00611-6.
Ururahy MS, Curylofo-Zotti FA, Galo R, Nogueira LF, Ramos AP, Corona SA. Wettability and surface morphology of eroded dentin treated with chitosan. Arch Oral Biol. 2017 Mar;75:68-73. doi: 10.1016/j.archoralbio.2016.11.017.
Cvar JF, Ryge G. Reprint of criteria for the clinical evaluation of dental restorative materials. 1971. Clin Oral Investig. 2005 Dec;9(4):215-32. doi: 10.1007/s00784-005-0018-z.
Cruz JB, Bonini G, Lenzi TL, Imparato JCP, Raggio DP. Bonding stability of adhesive systems to eroded dentin. Dent Mater. 2015;29:S1806-83242015000100284. doi: 10.1590/1807-3107BOR-2015.vol29.0088.
Tjäderhane L, Buzalaf MAR, Carrilho M, Chaussain C. Matrix metalloproteinases and other matrix proteinases in relation to cariology: the era of ‘dentin degradomics’. Caries Res. 2015;49(3):193-208. doi: 10.1159/000363582.
Passos VF, Melo MAS, Lima JPM, Marçal FF, Costa CAGA, Rodrigues LKA, et al. Active compounds and derivatives of camellia sinensis responding to erosive attacks on dentin. Braz Oral Res. 2018 May;32:e40. doi: 10.1590/1807-3107bor-2018.vol32.0040.
Beltrame APCA, Suchyta D, Abd Alraheam I, Mohammed A, Schoenfisch M, Walter R, et al. Effect of phosphorylated chitosan on dentin erosion: an in vitro study. Caries Res. 2018;52(5):378-86. doi: 10.1159/000486521.
Souza BM, Machado PF, Vecchia LR, Magalhães AC. Effect of chitosan solutions with or without fluoride on the protection against dentin erosion in vitro. Eur J Oral Sci. 2020 Dec;128(6):495-500. doi: 10.1111/eos.12740.
Souza-Gabriel AE, Sousa-Neto MD, Scatolin RS, Corona SAM. Durability of resin on bleached dentin treated with antioxidant solutions or lasers. J Mech Behav Biomed Mater. 2020 Apr;104:103647. doi: 10.1016/j.jmbbm.2020.103647.
Macedo FAA, Souza NO, Lemos MVS, De-Paula DM, Santiago SL, Feitosa VP. Dentin bonding and physicochemical properties of adhesives incorporated with epigallocatechin-3-gallate. Odontology. 2019 Jan;107(1):23-28. doi: 10.1007/s10266-018-0367-0.
Van Landuyt KL, Peumans M, De Munck J, Lambrechts P, Van Meerbeer B. Extension of a one-step self-etch adhesive into a multi-step adhesive. Dent Mater. 2006 Jun;22(6):533-44. doi: 10.1016/j.dental.2005.05.010.
Muñoz MA, Luque I, Hass V, Reis A, Loguercio AD, Bombarda NH. Immediate bonding properties of universal adhesives to dentine. J Dent. 2013 May;41(5):404-11. doi: 10.1016/j.jdent.2013.03.001.
Perdigão J, Dutra-Corrêa M, Saraceni CH, Ciaramicoli MT, Kiyan VH, Queiroz CS. Randomized clinical trial of four adhesion strategies: 18-month results. Oper Dent. 2012 Jan-Feb;37(1):3-11. doi: 10.2341/11-222-C.
André CB, Nima G, Sebold M, Giannini M, Price RB. Stability of the light output, oral cavity tip accessibility in posterior region and emission spectrum of light-curing units. Oper Dent. 2018 Jul/Aug;43(4):398-407. doi: 10.2341/17-033-L.
Magalhães AC, Wiegand A, Rios D, Hannas A, Attin T, Buzalaf MA. Chlorhexidine and green tea extract reduce dentin erosion and abrasion in situ. J Dent. 2009 Dec;37(12):994-8. doi: 10.1016/j.jdent.2009.08.007.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2022 Renato Gonçalves dos Reis, Antônio Cláudio Tedesco, Fabiana Almeida Curylofo-Zotti, Thiago Vinicius Cortez, Hiago Salge Borges, Aline Evangelista Souza-Gabriel, Silmara Aparecida Milori Corona
Downloads
