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Effects of black tea tooth staining previously to 35% hydrogen peroxide bleaching


Bleaching agents
Hydrogen peroxide
Staining and labeling

How to Cite

Palandi S da S, Kury M, Picolo MZD, Esteban Florez FL, Cavalli V. Effects of black tea tooth staining previously to 35% hydrogen peroxide bleaching. Braz. J. Oral Sci. [Internet]. 2022 Sep. 13 [cited 2023 Sep. 26];22(00):e238082. Available from:


Aim: To determine if the artificial staining with black tea (BT) influences the enamel microhardness before in-office bleaching and if BT staining is necessary to evaluate the efficacy of bleaching with 35% hydrogen peroxide Methods: Enamel/dentin blocks were randomized into groups according to the staining protocol (n=5/group): (CO) control – maintained in artificial saliva solution (AS); (BT4) immersed in black tea solution for 4 h; (BT24) immersed in black tea solution for 24 h. After the staining protocols, all specimens were kept in AS for one week, followed by bleaching (three sessions of HP application for 40 min). Knoop surface microhardness (kgF/mm2) was determined at baseline (T0), after staining (T1), after 7 days of storage in AS (T2), and after bleaching (T3). The color (ΔE00) and coordinate changes (ΔL, Δa, Δb) were measured using a digital spectrophotometer at T0 and T3. Data were submitted to one-way (ΔE00, ΔL, Δa, Δb) or two-way ANOVA repeated measures (kgF/mm2) and Tukey’s test (a=5%). Results: The staining protocols (BT4 and BT24) promoted significantly lower microhardness (T1 and T2, p<0.05) than CO, whereas CO was the only group to maintain microhardness values over time. Bleaching promoted perceptible ΔE00 without a significant difference among the groups regardless of the staining protocol (p=0.122). CO and BT4 showed no differences in terms of ΔL and Δa (p>0.05), but BT4 displayed a higher Δb than CO. Conclusion: The artificial staining with BT negatively affected the enamel surface microhardness and was not essential to evaluate the efficacy of 35% hydrogen peroxide bleaching.


Addy M, Moran J, Newcombe R, Warren P. The comparative tea staining potential of phenolic, chlorhexidine and anti-adhesive mouthrinses. J Clin Periodontol. 1995 Dec;22(12):923-8. doi: 10.1111/j.1600-051x.1995.tb01796. x.

Rodríguez-Martínez J, Valiente M, Sánchez-Martín MJ. Tooth whitening: From the established treatments to novel approaches to prevent side effects. J Esthet Restor Dent. 2019;31(5):431-40. doi: 10.1111/jerd.12519.

Sulieman M, Addy M, Rees JS. Development and evaluation of a method in vitro to study the effectiveness of tooth bleaching. J Dent. 2003 Aug;31(6):415-22. doi: 10.1016/s0300-5712(03)00069-1.

Prathap S, Rajesh H, Boloor VA, Rao AS. Extrinsic stains and management: a new insight. J Acad Indus Res. 2013;1(8):435-42.

Żyła T, Kawala B, Antoszewska-Smith J, Kawala M. Black stain and dental caries: a review of the literature. Biomed Res Int. 2015; 2015:469392. doi: 10.1155/2015/469392.

de Geus JL, de Lara MB, Hanzen TA, Fernández E, Loguercio AD, Kossatz S, et al. One-year follow-up of at-home bleaching in smokers before and after dental prophylaxis. J Dent. 2015 Nov;43(11):1346-51. doi: 10.1016/j.jdent.2015.08.009.

Joiner A, Luo W. Tooth color and whiteness: a review. J Dent. 2017 Dec;67S:S3-S10. doi: 10.1016/j.jdent.2017.09.006.

Kwon SR, Wertz PW. Review of the mechanism of tooth whitening. J Esthet Restor Dent. 2015 Sep-Oct;27(5):240-57. doi: 10.1111/jerd.12152.

Llena C, Martínez-Galdón O, Forner L, Gimeno-Mallench L, Rodríguez-Lozano FJ, Gambini J. Hydrogen peroxide diffusion through enamel and dentin. Materials 2018;11(9):1694. doi: 10.3390/ma11091694.

Gallinari MO, Fagundes TC, da Silva LM, de Almeida Souza MB, Barboza A, Briso A. A new approach for dental bleaching using violet light with or without the use of whitening gel: study of bleaching effectiveness. Oper Dent. 2019 Sep-Oct;44(5):521-9. doi: 10.2341/17-257-L.

Kury M, Perches C, da Silva DP, André CB, Tabchoury CPM, Giannini M, et al. Color change, diffusion of hydrogen peroxide, and enamel morphology after in-office bleaching with violet light or nonthermal atmospheric plasma: an in vitro study. J Esthet Restor Dent. 2020 Jan;32(1):102-12. doi: 10.1111/jerd.12556.

Prskalo K, Klarić Sever E, Alerić I, Antonić Jelić T, Žaja I. Risk factors associated with black tooth stain. Acta Clin Croat. 2017 Mar;56(1):28-35. doi: 10.20471/acc.2017.56.01.05.

Kobayashi RS, Picolo MZD, Kury M, Resende BA, Esteban Florez FL, Cavalli V. Effects of dental bleaching protocols with violet radiation on the color and chemical composition of stained bovine enamel. Photodiagnosis Photodyn Ther. 2021 Jun;34:102194. doi: 10.1016/j.pdpdt.2021.102194.

Berger SB, Coelho AS, Oliveira VA, Cavalli V, Giannini M. Enamel susceptibility to red wine staining after 35% hydrogen peroxide bleaching. J Appl Oral Sci. 2008 May-Jun;16(3):201-4. doi: 10.1590/s1678-77572008000300007.

Téo TB, Takahashi MK, Gonzaga CC, Lopes MGK. Avaliação, após clareamento, da alteração de cor de dentes bovinos imersos em soluções com elevado potencial de pigmentação. Rev Sul-Bras Odontol. 2010 Oct-Dec;7(4):401-5.

de Oliveira Duque CC, Soares DG, Briso A, Ortecho-Zuta U, de Oliveira Ribeiro RA, Hebling J, et al. Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching. Oper Dent. 2020 Nov;45(6):632-42. doi: 10.2341/19-013-L.

Cury JA. [Fluoride use and caries control as a disease]. In: Baratieri LN, et al. [Restorative dentistry: fundamentals and possibilities]. São Paulo: Santos; 2001. p.31-68. Portuguese.

Lun Su Y, Leung LK, Huang Y, Chen Z-Y. Stability of tea theaflavins and catechins. Food Chem. 2003;83(2):189-95. doi:10.1016/s0308-8146(03)00062-1.

Cavalli V, Rosa DAD, Silva DPD, Kury M, Liporoni PCS, Soares LES, et al. Effects of experimental bleaching agents on the mineral content of sound and demineralized enamels. J Appl Oral Sci. 2018 Oct; 26: e20170589. doi: 10.1590/1678-7757-2017-0589.

Zantner C, Beheim-Schwarzbach N, Neumann K, Kielbassa AM. Surface microhardness of enamel after different home bleaching procedures. Dent Mater. 2007 Feb;23(2):243-50. doi: 10.1016/

Queiroz CS, Hara AT, Paes Leme AF, Cury JA. pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de and remineralization. Braz Dent J 2008;19(1):21-7 doi: 10.1590/S0103-64402008000100004.

Costa JLSG, Besegato JF, Kuga MC. Bleaching and microstructural effects of low concentration hydrogen peroxide photoactivated with LED/laser system on bovine enamel. Photodiagnosis Photodyn Ther. 2021 Sep;35:102352. doi: 10.1016/j.pdpdt.2021.102352.

Paravina RD, Pérez MM, Ghinea R. Acceptability, and perceptibility thresholds in dentistry: A comprehensive review of clinical and research applications. J Esthet Restor Dent. 2019 Mar;31(2):103-12. doi: 10.1111/jerd.12465 .

Thacker M, Chen YN, Lin CP, Lin FH. Nitrogen-doped titanium dioxide mixed with calcium peroxide and methylcellulose for dental bleaching under visible light activation. Int J Mol Sci. 2021 Apr;22(7):3759. doi: 10.3390/ijms22073759.

Ferraz NKL, Nogueira LC, Neiva IM, Ferreira RC, Moreira AN, Magalhães CS. Longevity, effectiveness, safety, and impact on quality of life of low-concentration hydrogen peroxides in-office bleaching: a randomized clinical trial. Clin Oral Investig. 2019 May;23(5):2061-70. doi: 10.1007/s00784-018-2607-7.

Kyaw KY, Otsuki M, Segarra MS, Tagami J. Effect of sodium fluoride pretreatment on the efficacy of an in-office bleaching agent: an in vitro study. Clin Exp Dent Res. 2018 Jun 29;4(4):113-8. doi: 10.1002/cre2.113.

Costa JLSG, Besegato JF, Zaniboni JF, Kuga MC. LED/laser photoactivation enhances the whitening efficacy of low concentration hydrogen peroxide without microstructural enamel changes. Photodiagnosis Photodyn Ther. 2021 Dec;36:102511. doi: 10.1016/j.pdpdt.2021.102511.

Simpson A, Shaw L, Smith AJ. Tooth surface pH during drinking of black tea. Br Dent J. 2001 Apr;190(7):374-6. doi: 10.1038/sj.bdj.4800977.

Jameel RA, Khan SS, Abdul Rahim ZH, Bakri MM, Siddiqui S. Analysis of dental erosion induced by different beverages and validity of equipment for identifying early dental erosion, in vitro study. J Pak Med Assoc. 2016 Jul;66(7):843-8.

Farawati FAL, Hsu SM, O'Neill E, Neal D, Clark A, Esquivel-Upshaw J. Effect of carbamide peroxide bleaching on enamel characteristics and susceptibility to further discoloration. J Prosthet Dent. 2019 Feb;121(2):340-6. doi: 10.1016/j.prosdent.2018.03.006.

Hayacibara MF, Queiroz CS, Tabchoury CPM, Cury JA. Fluoride and aluminum in teas and tea-based beverages. Rev Saude Publica. 2004 Feb;38(1):100-5. doi: 10.1590/s0034-89102004000100014.

Amoras DR, Corona SAM, Rodrigues Jr AL, Serra MC. Effect of beverages on bovine dental enamel subjected to erosive challenge with hydrochloric acid. Braz Dent J. 2012;23(4):367-72. doi: 10.1590/S0103-64402012000400010.

Kury M, Wada EE, Silva DPD, Tabchoury CPM, Giannini M, Cavalli V. Effect of violet LED light on in-office bleaching protocols: a randomized controlled clinical trial. J Appl Oral Sci. 2020;28:e20190720. doi: 10.1590/1678-7757-2019-0720.

Kury M, Wada EE, da Silva Palandi S, Picolo MZD, Giannini M, Cavalli V. Colorimetric evaluation after in-office tooth bleaching with a violet LED: 6- and 12-month follow-ups of a randomized clinical trial. Clin Oral Investig. 2022 Jan;26(1):837-47 16. doi: 10.1007/s00784-021-04062-9.

Sharma G, Wu W, Dalal EN. The CIEDE2000 color-difference formula: Implementation notes, supplementary test data, and mathematical observations. Color Res Appl. 2005 Feb;30(1):21-30. doi: 10.1002/col.20070.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2022 Samuel da Silva Palandi, Matheus Kury, Mayara Zaghi Dal Picolo, Fernando Luis Esteban Florez, Vanessa Cavalli


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