Effects of black tea tooth staining previously to 35% hydrogen peroxide bleaching





Bleaching agents, Hydrogen peroxide, Staining and labeling


Aim: To determine if the artificial staining with black tea (BT) influences the enamel microhardness before in-office bleaching and if BT staining is necessary to evaluate the efficacy of bleaching with 35% hydrogen peroxide Methods: Enamel/dentin blocks were randomized into groups according to the staining protocol (n=5/group): (CO) control – maintained in artificial saliva solution (AS); (BT4) immersed in black tea solution for 4 h; (BT24) immersed in black tea solution for 24 h. After the staining protocols, all specimens were kept in AS for one week, followed by bleaching (three sessions of HP application for 40 min). Knoop surface microhardness (kgF/mm2) was determined at baseline (T0), after staining (T1), after 7 days of storage in AS (T2), and after bleaching (T3). The color (ΔE00) and coordinate changes (ΔL, Δa, Δb) were measured using a digital spectrophotometer at T0 and T3. Data were submitted to one-way (ΔE00, ΔL, Δa, Δb) or two-way ANOVA repeated measures (kgF/mm2) and Tukey’s test (a=5%). Results: The staining protocols (BT4 and BT24) promoted significantly lower microhardness (T1 and T2, p<0.05) than CO, whereas CO was the only group to maintain microhardness values over time. Bleaching promoted perceptible ΔE00 without a significant difference among the groups regardless of the staining protocol (p=0.122). CO and BT4 showed no differences in terms of ΔL and Δa (p>0.05), but BT4 displayed a higher Δb than CO. Conclusion: The artificial staining with BT negatively affected the enamel surface microhardness and was not essential to evaluate the efficacy of 35% hydrogen peroxide bleaching.


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Author Biographies

Samuel da Silva Palandi, University of Campinas

Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas.

Matheus Kury, University of Campinas

Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas.

Mayara Zaghi Dal Picolo, University of Campinas

Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas.

Fernando Luis Esteban Florez, Oklahoma University Health Sciences Center

Department of Restorative Sciences, Division of Dental Materials, College of Dentistry, Oklahoma University Health Sciences Center.

Vanessa Cavalli, University of Campinas

Department of Restorative Dentistry, Piracicaba Dental School, University of Campinas.


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How to Cite

Palandi S da S, Kury M, Picolo MZD, Esteban Florez FL, Cavalli V. Effects of black tea tooth staining previously to 35% hydrogen peroxide bleaching. Braz. J. Oral Sci. [Internet]. 2022 Sep. 13 [cited 2022 Dec. 5];22(00):e238082. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8668082



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