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Effect of phenylmethylsulfonyl fluoride, a protease inhibitor, on enamel surface remineralization
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Keywords

Protease inhibitor
Dental enamel
Fluorides
Tooth remineralization

How to Cite

1.
Peres PEC, Fu J, Zero DT, Cury JA. Effect of phenylmethylsulfonyl fluoride, a protease inhibitor, on enamel surface remineralization. Braz. J. Oral Sci. [Internet]. 2022 Nov. 3 [cited 2024 Apr. 15];22(00):e230883. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8670883

Abstract

Phenylmethylsulfonyl fluoride (PMSF) is a protease inhibitor widely used in research, but fluoride is released during its action and this knowledge has been neglected in dental research. Aim: to evaluate if fluoride released by salivary protease action on PMSF affects enamel remineralization and fluoride uptake. Methods: Groups of 10 enamel slabs, with caries-like lesions and known surface hardness (SH), were subjected to one of the following treatment groups: Stimulated human saliva (SHS), negative control; SHS containing 1.0 μg F/mL (NaF), positive control; and SHS containing 10, 50 or 100 μM PMSF. The slabs were subjected to a pH-cycling regimen consisting of 22 h/day in each treatment solution and 2 h/day in a demineralizing solution. After 12 days, SH was again measured to calculate the percentage of surface hardness recovery (%SHR), followed by enamel fluoride uptake determination. The time-related fluoride release from 100.0 μM PMSF by SHS action was also determined. Data were analyzed by ANOVA followed by Newman-Keuls test. Results: The release of fluoride from PMSF by SHS was rapid, reaching a maximum value after 10 min. Fluoride released from PMSF was more effective in enhancing %SHR and increasing fluoride uptake in enamel compared with SHS alone (p < 0.05); furthermore, it was equivalent to the positive control (p > 0.05). Conclusion: In conclusion, fluoride released by saliva from PMSF is available to react with enamel and needs to be taken into account in research using this protease inhibitor.

https://doi.org/10.20396/bjos.v22i00.8670883
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References

Papale F, Santonocito S, Polizzi A, Giudice AL, Capodiferro S, Favia G, et al. The new era of salivaomics in dentistry: frontiers and facts in the early diagnosis and prevention of oral diseases and cancer. Metabolites. 2022 Jul;12(7):638. doi: 10.3390/metabo12070638.

Siqueira WL, Dawes C. The salivary proteome: challenges and perspectives. Proteomics Clin Appl. 2011 Dec;5(11-12):575-9. doi: 10.1002/prca.201100046.

Xiao H, Wong DT. Method development for proteome stabilization in human saliva. Anal Chim Acta. 2012 Apr;722:63-9. doi: 10.1016/j.aca.2012.02.017.

Fahrney DE, Gold AM. Sulfonyl Fluorides as Inhibitor of Esterases. I. Rates of Reaction with Acetylcholinesterase, α-Chymotrypsin, and Trypsin, J Am Chem Soc. 1963;85(7):997-1000. doi: 10.1021/ja00890a037.

Minaguchi K, Madapallimattam G, Bennick A. The presence and origin of phosphopeptides in human saliva. Biochem J. 1988 Feb;250(1):171-7. doi: 10.1042/bj2500171.

Nieuw Amerongen AV, Oderkerk CH, Driessen AA. Role of mucins from human whole saliva in the protection of tooth enamel against demineralization in vitro. Caries Res. 1987;21(4):297-309. doi: 10.1159/000261033.

Fisher SJ, Prakobphol A, Kajisa L, Murray PA. External radiolabelling of components of pellicle on human enamel and cementum. Arch Oral Biol. 1987;32(7):509-17. doi: 10.1016/s0003-9969(87)80013-4.

Koller MM, Maeda N, Purushotham KR, Scarpace PJ, Humphreys-Beher MG. A biochemical analysis of parotid and submandibular salivary gland function with age after simultaneous stimulation with pilocarpine and isoproterenol in female NIA Fischer 344 rats. Arch Oral Biol. 1992 Mar;37(3):219-30. doi: 10.1016/0003-9969(92)90092-m.

Steinberg D, Beeman D, Bowen WH. The effect of delmopinol on glucosyltransferase adsorbed on to saliva-coated hydroxyapatite. Arch Oral Biol. 1992 Jan;37(1):33-8. doi: 10.1016/0003-9969(92)90150-7.

Stinson MW, Haraszthy GG, Zhang XL, Levine MJ. Inhibition of Porphyromonas gingivalis adhesion to Streptococcus gordonii by human submandibular-sublingual saliva. Infect Immun. 1992 Jul;60(7):2598-604. doi: 10.1128/iai.60.7.2598-2604.1992.

Iontcheva I, Oppenheim FG, Troxler RF. Human salivary mucin MG1 selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins. J Dent Res. 1997 Mar;76(3):734-43. doi: 10.1177/00220345970760030501.

Bassim CW, Ambatipudi KS, Mays JW, Edwards DA, Swatkoski S, Fassil H, et al. Quantitative salivary proteomic differences in oral chronic graft-versus-host disease. J Clin Immunol. 2012 Dec;32(6):1390-9. doi: 10.1007/s10875-012-9738-4.

Rölla G, Melsen B. Desorption of protein and bacteria from hydroxyapatite by fluoride and monofluorophosphate. Caries Res. 1975;9(1):66-73. doi: 10.1159/000260144.

Hamilton IR. Biochemical effects of fluoride on oral bacteria. J Dent Res. 1990 Feb;69 Spec No:660-7; discussion 682-3. doi: 10.1177/00220345900690S128.

Bradshaw DJ, Marsh PD, Hodgson RJ, Visser JM. Effects of glucose and fluoride on competition and metabolism within in vitro dental bacterial communities and biofilms. Caries Res. 2002 Mar-Apr;36(2):81-6. doi: 10.1159/000057864.

Cury JA, Tenuta LM. Enamel remineralization: controlling the caries disease or treating early caries lesions? Braz Oral Res. 2009;23 Suppl 1:23-30. doi: 10.1590/s1806-83242009000500005.

Zero DT. In situ caries models. Adv Dent Res. 1995 Nov;9(3):214-30; discussion 231-4. doi: 10.1177/08959374950090030501.

White DJ. Reactivity of fluoride dentifrices with artificial caries. I. Effects on early lesions: F uptake, surface hardening and remineralization. Caries Res. 1987;21(2):126-40. doi: 10.1159/000261013.

Koo RH, Cury JA. Soluble calcium/SMFP dentifrice: effect on enamel fluoride uptake and remineralization. Am J Dent. 1998 Aug;11(4):173-6.

Fiske CM, Subarrow Y. The colorimetric determination of phosphorus. J Biol Chem. 1925 Dec;66(2):375-400.

Ayres M, Ayres M Jr, Ayres DL, Santos AS: BioEstat 2.0: Statistical Applications in Biological Sciences and Medicine. Belém: Sociedade Civil Mamiraua; 2000. Portuguese.

Feng Y, Li Q, Chen J, Yi P, Xu X, Fan Y, et al. Salivary protease spectrum biomarkers of oral cancer. Int J Oral Sci. 2019 Jan;11(1):7. doi: 10.1038/s41368-018-0032-z.

Shellis RP, Duckworth RM. Studies on the cariostatic mechanisms of fluoride. Int Dent J. 1994 Jun;44(3 Suppl 1):263-73.

Pearce EIF, Jenkins GN: The decomposition of monofluorophosphate by enzymes in whole human saliva. Arch Oral Biol 1977;22(6):405-7. doi: 10.1016/0003-9969(77)90064-4.

Bowen WH. Relative efficacy of sodium fluoride and sodium monofluorophosphate as anti-caries agents in dentifrices: Proceedings of a conference sponsored by Unilever Research, held at the Royal Society of Medicine, London, 5 November 1994. London: Royal Society of Medicine Press; 1995. 66p.

Tenuta LMA, Cury JA. Laboratory and human studies to estimate anticaries efficacy of fluoride toothpastes. Monogr Oral Sci. 2013;23:108-24. doi: 10.1159/000350479.

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Copyright (c) 2022 Paulo Edelvar Corrêa Peres, Jean Fu, Zero DT, Jaime Aparecido Cury

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