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ENAM gene polymorphisms associated with dental anomalies in individuals with cleft lip and palate
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Keywords

Cleft lip
Cleft palate
Extracellular matrix proteins
Polymorphism
Single nucleotide

How to Cite

1.
Oliveira FV de, Santos CF dos, Dionísio TJ, Neves LT das, Dalben G da S, Ambrosio ECP, et al. ENAM gene polymorphisms associated with dental anomalies in individuals with cleft lip and palate. Braz. J. Oral Sci. [Internet]. 2024 May 3 [cited 2024 Jun. 16];23(00):e241330. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8671330

Abstract

Aim: This study aimed to investigate the occurrence of enamelin gene (ENAM) single nucleotide polymorphisms (SNP) and ENAM polymorphism association with dental anomalies (DA) in individuals with unilateral or bilateral cleft lip and palate (CLP). Methods: Saliva samples were collected from 147 individuals aged between 6 and 15 years-old, both genders, and divided into 4 groups: Group 1 (G1) - CLP and DA; Group 2 (G2) - CLP without DA; Group 3 (G3) - without CLP with DA; Group 4 (G4) - without CLP and DA. The genomic DNA was extracted from saliva samples and the following ENAM SNPs markers were genotyped: rs3796703, rs3796704, rs3796705, rs7671281, rs2609428, and rs35951442. Fisher exact and Pearson’s Chi-square tests statistically analyzed the results (α=5%). Results: Individuals without CLP with DA (Group 3 - 19.2%) showed statistically higher prevalence of SNP rs2609428 heterozygotes (p=0.006) than individuals with CLP and DA (Group 1 - 0%). Individuals without CLP (10%) exhibited statistically higher prevalence of mutated heterozygotes/homozygous (p=0.028) than in individuals with CLP (1.3%). Conclusion: SNP rs2609428 marker of ENAM gene may be associated with dental anomalies in individuals without cleft lip and palate.

https://doi.org/10.20396/bjos.v23i00.8671330
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Copyright (c) 2024 Fernanda Veronese de Oliveira, Carlos Ferreira dos Santos, Thiago José Dionísio, Lucimara Teixeira das Neves, Gisele da Silva Dalben, Eloá Cristina Passucci Ambrosio, Paula Karine Jorge, Maria Aparecida Andrade Moreira Machado, Thais Marchini Oliveira

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