Keywords
Calcium compounds
Sodium hypochlorite
Root canal obturation
How to Cite
Funding data
-
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Grant numbers 001
Abstract
Aim: This study aimed to compare the influence of sodium hypochlorite (NaOCl) and calcium hypochlorite [Ca(OCl)2 ] on the apical sealing capacity of AH Plus (Dentsply Maillefer) and Bio-C Sealer (Angelus) endodontic sealers. Methods: Sixty permanent human lower incisors were randomly allocated (http://www.randomized.org), according to the irrigant used, into three groups (n=20): 0.9% sodium chloride (NaCl/Control); 2.5% NaOCl; and 2.5% Ca(OCl)2 . The root canal was prepared with rotary files under 10 mL of the solution corresponding to the experimental group. Each group was subdivided into two (n=10) according to the sealer used for filling: AH Plus (Dentsply Maillefer) or Bio-C Sealer (Angelus). Then, all samples were immersed in black India ink for one week. After the storage period, the roots were then grooved longitudinally and split, and the ink penetration was measured from the apical part to the coronal part of the root canal into which the ink penetrated using a stereomicroscope. Data were analyzed by one-way ANOVA and Tukey’s post-hoc tests. Results: There was no statistical difference in ink penetration between the different endodontic sealers tested for the same irrigating solution (p > 0.05). However, when the Bio-C Sealer (Angelus) was used, the group treated with 2.5% Ca(OCl)2 was associated with lower values of apical leakage, compared to 2.5% NaOCl (p < 0.05). For the AH Plus sealer (Dentsply Maillefer), there was no difference between the irrigants (p > 0.05). Conclusions: Associating Ca(OCl)2 irrigant with Bio-C Sealer (Angelus) seems to be a good option to reduce apical leakage.
References
Peters OA, Schönenberger K, Laib A. Effects of four Ni-Ti preparation techniques on root canal geometry assessed by micro computed tomography. Int Endod J. 2001 Apr;34(3):221-30. doi: 10.1046/j.1365-2591.2001.00373.x.
Haapasalo M, Shen Y, Wang Z, Gao Y. Irrigation in endodontics. Br Dent J. 2014 Mar;216(6):299-303. doi: 10.1038/sj.bdj.2014.204.
Zehnder M. Root canal irrigants. J Endod. 2006 May;32(5):389-98. doi: 10.1016/j.joen.2005.09.014.
Ishihara M, Murakami K, Fukuda K, Nakamura S, Kuwabara M, Hattori H, et al. Stability of weakly acidic hypochlorous acid solution with microbicidal activity. Biocontrol Sci. 2017;22(4):223-7. doi: 10.4265/bio.22.223.
Sauro S, Mannocci F, Tay FR, Pashley DH, Cook R, Carpenter GH, et al. Deproteinization effects of NaOCl on acid-etched dentin in clinically-relevant vs prolonged periods of application. A confocal and environmental scanning electron microscopy study. Oper Dent. 2009 Mar-Apr;34(2):166-73. doi: 10.2341/08-56.
Leonardo NG, Carlotto IB, Luisi SB, Kopper PM, Grecca FS, Montagner F. Calcium hypochlorite solutions: evaluation of surface tension and effect of different storage conditions and time periods over pH and available chlorine content. J Endod. 2016 Apr;42(4):641-5. doi: 10.1016/j.joen.2016.01.006.
Blattes GB, Mestieri LB, Böttcher DE, Fossati AC, Montagner F, Grecca FS. Cell migration, viability and tissue reaction of calcium hypochlorite based-solutions irrigants: an in vitro and in vivo study. Arch Oral Biol. 2017 Jan;73:34-39. doi: 10.1016/j.archoralbio.2016.08.037.
Paula KB, Carlotto IB, Marconi DF, Ferreira MBC, Grecca FS, Montagner F. Calcium hypochlorite solutions - an in vitro evaluation of antimicrobial action and pulp dissolution. Eur Endod J. 2019 Jan 10;4(1):15-20. doi: 10.14744/eej.2018.64936.
Dutta A, Saunders WP. Comparative evaluation of calcium hypochlorite and sodium hypochlorite on soft-tissue dissolution. J Endod. 2012 Oct;38(10):1395-8. doi: 10.1016/j.joen.2012.06.020.
Cecchin D, Soares Giaretta V, Granella Cadorin B, Albino Souza M, Vidal CMP, Paula Farina A. Effect of synthetic and natural-derived novel endodontic irrigant solutions on mechanical properties of human dentin. J Mater Sci Mater Med. 2017 Aug;28(9):141. doi: 10.1007/s10856-017-5960-1.
Durigon M, Cecchin D, de Carli JP, Souza MA, Farina AP. Could calcium hypochlorite and grape seed extract keep the mechanical properties of root dentin and fracture resistance of weakened roots? J Mech Behav Biomed Mater. 2020 Jun;106:103736. doi: 10.1016/j.jmbbm.2020.103736.
McMichen FR, Pearson G, Rahbaran S, Gulabivala K. A comparative study of selected physical properties of five root-canal sealers. Int Endod J. 2003 Sep;36(9):629-35. doi: 10.1046/j.1365-2591.2003.00701.x.
Paqué F, Sirtes G. Apical sealing ability of Resilon/Epiphany versus gutta-percha/AH Plus: immediate and 16-months leakage. Int Endod J. 2007 Sep;40(9):722-9. doi: 10.1111/j.1365-2591.2007.01298.x.
Giacomino CM, Wealleans JA, Kuhn N, Diogenes A. Comparative biocompatibility and osteogenic potential of two bioceramic sealers. J Endod. 2019 Jan;45(1):51-56. doi: 10.1016/j.joen.2018.08.007.
Alves Silva EC, Tanomaru-Filho M, da Silva GF, Delfino MM, Cerri PS, Guerreiro-Tanomaru JM. Biocompatibility and Bioactive Potential of New Calcium Silicate-based Endodontic Sealers: Bio-C Sealer and Sealer Plus BC. J Endod. 2020 Oct;46(10):1470-7. doi: 10.1016/j.joen.2020.07.011.
Zanza A, Reda R, Vannettelli E, Donfrancesco O, Relucenti M, Bhandi S, et al al. The influence of thermomechanical compaction on the marginal adaptation of 4 different hydraulic sealers: a comparative ex vivo study. J Compos Sci. 2023;7(1):1-13. doi: 10.3390/jcs7010010.
Sagsen B, Ustün Y, Demirbuga S, Pala K. Push-out bond strength of two new calcium silicate-based endodontic sealers to root canal dentine. Int Endod J. 2011 Dec;44(12):1088-91. doi: 10.1111/j.1365-2591.2011.01925.x.
Zordan-Bronzel CL, Esteves Torres FF, Tanomaru-Filho M, Chávez-Andrade GM, Bosso-Martelo R, Guerreiro-Tanomaru JM. Evaluation of Physicochemical Properties of a New Calcium Silicate-based Sealer, Bio-C Sealer. J Endod. 2019 Oct;45(10):1248-52. doi: 10.1016/j.joen.2019.07.006.
Gillen BM, Looney SW, Gu LS, Loushine BA, Weller RN, Loushine RJ, et al. Impact of the quality of coronal restoration versus the quality of root canal fillings on success of root canal treatment: a systematic review and meta-analysis. J Endod. 2011 Jul;37(7):895-902. doi: 10.1016/j.joen.2011.04.002.
Mohamed EA, Fathieh SM, Farzaneh TA, Homeira BA. Effect of Different irrigation solutions on the apical sealing ability of different single-cone obturation systems: an in vitro study. J Contemp Dent Pract. 2019 Feb 1;20(2):158-65.
Bodrumlu E, Parlak E, Bodrumlu EH. The effect of irrigation solutions on the apical sealing ability in different root canal sealers. Braz Oral Res. 2010 Apr-Jun;24(2):165-9. doi: 10.1590/s1806-83242010000200007.
Antunes TBM, Janini ACP, Pelepenko LE, Abuna GF, Paiva EM, Sinhoreti MAC, et al. Heating stability, physical and chemical analysis of calcium silicate-based endodontic sealers. Int Endod J. 2021 Jul;54(7):1175-88. doi: 10.1111/iej.13496.
Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of irrigation sequence on the adhesion of root canal sealers to dentin: a fourier transform infrared spectroscopy and push-out bond strength analysis. J Endod. 2015 Jul;41(7):1108-11. doi: 10.1016/j.joen.2015.02.001.
Neelakantan P, Varughese AA, Sharma S, Subbarao CV, Zehnder M, De-Deus G. Continuous chelation irrigation improves the adhesion of epoxy resin-based root canal sealer to root dentine. Int Endod J. 2012 Dec;45(12):1097-102. doi: 10.1111/j.1365-2591.2012.02073.x.
Huang Y, Orhan K, Celikten B, Orhan AI, Tufenkci P, Sevimay S. Evaluation of the sealing ability of different root canal sealers: a combined SEM and micro-CT study. J Appl Oral Sci. 2018 Jan;26:e20160584. doi: 10.1590/1678-7757-2016-0584.
Ersahan S, Aydin C. Solubility and apical sealing characteristics of a new calcium silicate-based root canal sealer in comparison to calcium hydroxide-, methacrylate resin- and epoxy resin-based sealers. Acta Odontol Scand. 2013 May-Jul;71(3-4):857-62. doi: 10.3109/00016357.2012.734410.
Ferreira MB, Carlini Júnior B, Galafassi D, Gobbi DL. Calcium hypochlorite as a dentin deproteinization agent: Microleakage, scanning electron microscopy and elemental analysis. Microsc Res Tech. 2015 Aug;78(8):676-81. doi: 10.1002/jemt.22524.
López-García S, Pecci-Lloret MR, Guerrero-Gironés J, Pecci-Lloret MP, Lozano A, Llena C, et al. Comparative Cytocompatibility and Mineralization Potential of Bio-C Sealer and TotalFill BC Sealer. Materials (Basel). 2019 Sep;12(19):3087. doi: 10.3390/ma12193087.
Pascon FM, Kantovitz KR, Sacramento PA, Nobre-dos-Santos M, Puppin-Rontani RM. Effect of sodium hypochlorite on dentine mechanical properties. A review. J Dent. 2009 Dec;37(12):903-8. doi: 10.1016/j.jdent.2009.07.004.
Görduysus M, Küçükkaya S, Bayramgil NP, Görduysus MÖ. Evaluation of the effects of two novel irrigants on intraradicular dentine erosion, debris and smear layer removal. Restor Dent Endod. 2015 Aug;40(3):216-22. doi: 10.5395/rde.2015.40.3.216.
Nessem R, Hassan HY, Fayyad DM. Effect of different root canal irrigating solutions on the apical sealing ability of bioceramic and resin-based sealers: an in vitro study. Dent Sci Update. 2023;4(1):83-96. doi: 10.21608/dsu.2023.132225.1123.
Sousa-Neto MD, Passarinho-Neto JG, Carvalho-Júnior JR, Cruz-Filho AM, Pécora JD, Saquy PC. Evaluation of the effect of EDTA, EGTA and CDTA on dentin adhesiveness and microleakage with different root canal sealers. Braz Dent J. 2002;13(2):123-8. doi: 10.1590/s0103-64402002000200009.
Veríssimo DM, do Vale MS. Methodologies for assessment of apical and coronal leakage of endodontic filling materials: a critical review. J Oral Sci. 2006 Sep;48(3):93-8. doi: 10.2334/josnusd.48.93.
Schäfer E, Olthoff G. Effect of three different sealers on the sealing ability of both thermafil obturators and cold laterally compacted Gutta-Percha. J Endod. 2002 Sep;28(9):638-42. doi: 10.1097/00004770-200209000-00003.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2024 Israel Bangel Carlotto, Natália Franco Brum, Guilherme Pauletto, Lucas Saldanha da Rosa, Carlos Alexandre Souza Bier
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