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Mango peel as a potential enzyme inducer in Trichoderma harzianum
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Keywords

Biofilms
Biotechnology
Glucans
Mangífera
Waste management

How to Cite

1.
Bem JSP, Polizello ACM, Cabral H, Rosa-Garzon NG da, Rechia CGV, Aires CP. Mango peel as a potential enzyme inducer in Trichoderma harzianum: a strategy for cariogenic biofilm degradation and reuse of industrial waste. Braz. J. Oral Sci. [Internet]. 2023 Nov. 21 [cited 2024 Jun. 26];22(00):e232955. Available from: https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8672955

Funding data

Abstract

Water-insoluble exopolysaccharides (I-EPS) are a virulence factor for dental biofilms. It has already been demonstrated that mango pulp induces the secretion of glucan-hydrolytic enzymes in the fungus Trichoderma harzianum, and that they have an effect on I-EPS from young biofilms. Aim: Evaluate the effect of mango peel as an enzyme inducer in T. harzianum, and the effect of enzymes secreted on mature biofilms. Methods: Fractions of the peel (PL) and ethanol-precipitated pulp (PP) of Tommy Atkins mangoes were sterilized and added to a culture medium containing T. harzianum for induction of hydrolytic enzymes. After 192 h, the culture medium was centrifuged and the supernatant (enzyme extract) was used as treatment on S. mutans biofilms (n=9): a) NaCl 0.9 %; b) 0.12 % chlorhexidine digluconate; and c) extract of enzymes induced by PL or PP. Acidogenicity, bacterial viability, quantification of insoluble polysaccharides, and three-dimensional analysis of the biofilm by scanning electron microscopy (SEM) was performed. Data were analyzed by ANOVA followed by the Tukey test (α=5 %). Results: The hydrolytic enzymes did not alter the metabolism or bacterial viability of the biofilm (p<0.05). Although the images obtained by SEM suggest some degree of matrix degradation, the quantification of I-EPS for the PL and PP groups did not differ from the control group (p>0.05), suggesting a slight effect on the disorganization of the mature S. mutans biofilm. Conclusion: The results suggest that mango peel fraction can induce secretion of mutanase by T. harzianum, however in an insufficient amount to generate significant degradation on cariogenic biofilm.

https://doi.org/10.20396/bjos.v22i00.8672955
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Copyright (c) 2022 Jéssica Silva Peixoto Bem, Ana Cristina Morseli Polizello, Hamilton Cabral, Nathalia Gonsales da Rosa-Garzon, Carem Gledes Vargas Rechia, Carolina Patrícia Aires

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