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Histomorphometric comparison of two different preclinical models to evaluate the bone repair in grafted areas


Animal experimentation
Bone regeneration
Bone substitutes

How to Cite

Lima JR, Soares PBF, Pereira L de SG, Rodríguez Perdomo L, Pigossi SC, Oliveira GJPL de. Histomorphometric comparison of two different preclinical models to evaluate the bone repair in grafted areas. Braz. J. Oral Sci. [Internet]. 2024 Jun. 3 [cited 2024 Jul. 12];23(00):e243937. Available from:


Aim: This study was performed to compare two different rat defect models (critical calvaria defects versus guided bone regeneration in the mandibular ramus) used to evaluate bone repair in grafted areas. Methods: A total of 12 rats were allocated in two groups according the experimental model used to evaluate the bone repair in grafted areas: a critical sized-calvaria defect of 5 mm filled with bone graft (n=6) and a mandibular ramus filled with the bone graft associated with a Teflon dome-shaped membrane (n=6). Both groups were grafted with deproteinized bovine bone graft. After 60 days, the animals were euthanized and the samples obtained were submitted to histomorphometry analysis to evaluate the relative amount of bone, remaining bone substitute, and soft tissue within the grafted areas. Results: No differences were observed between the preclinical models evaluated in relation to the amount of bone tissue formation (19.93 ± 4.55% in calvaria vs. 21.00 ± 8.20% in mandible). However, there was a smaller amount of soft tissue (43.20 ± 10.97% vs. 57.79 ± 7.61 %; p<0.01) and a greater amount of bone substitute remaining (35.80 ± 5.52% vs. 22.28 ± 4.36 %; p<0.05) in the grafted areas in the mandible compared to calvaria defect. Conclusion: Preclinical models for the analysis of bone repair in grafted areas in the mandible and critical sized-calvaria defects showed different responses in relation to the amount of soft tissue and bone substitute remnants.


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Copyright (c) 2024 Julia Raulino Lima, Priscilla Barbosa Ferreira Soares, Lucas de Souza Goulart Pereira , Leidys Rodríguez Perdomo, Suzane Cristina Pigossi, Guilherme José Pimentel Lopes de Oliveira


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